MR MICROSCOPY OF BRAIN DEVELOPMENT IN SEPSIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 脓毒症是一种潜在的危及生命的疾病，免疫系统对感染的反应可能会损害远离原始感染的身体组织。新生儿败血症在极低出生体重(VLBW)婴儿中尤其危险。研究表明，存活超过3天的极低出生体重婴儿中，约有21%的血培养证实为迟发性败血症。即使婴儿存活下来，脓毒症的不良影响也可能是长期的，导致大脑发育异常。然而，对新生儿进行准确的早期诊断是困难的，因为没有明确的诊断测试；即使是血培养也具有令人无法接受的低敏感性。因此，临床医生必须接受一些新生儿将开始治疗没有这种疾病的败血症。为了快速治疗所有患有脓毒症的婴儿，并尽量减少对未感染婴儿的治疗，需要改进技术来检测脓毒症的发病并研究其对发育的长期影响。这个试点项目将使用新开发的小鼠模型来测试和识别基于MRI的方法，以检测粪便感染引起的脑损伤。 目的1：我们将使用高分辨率定量技术来量化脓毒症引起的髓鞘形成的变化。 磁化率成像。 目标2：我们将用高分辨率量化白质完整性和连接性的退化 扩散张量成像。 该项目将评估两种有希望的MRI方法，用于新生儿败血症引起的脑白质损伤的早期诊断。成功的早期检测感染可以进行早期干预，防止对神经发育的潜在损害。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Sepsis is a potentially life-threatening condition, in which immune system's reaction to an infection may injure body tissues far from the original infection. Neonatal sepsis is particularly dangerous in very low birth weight (VLBW) infants. Studies have shown that about 21% of VLBW infants who survied beyond 3 days have blood culture proven late-onset sepsis. Even if the infant survives, the adverse effect of sepsis can be long lasting, resulting in abnormal brain development. However, accurate early diagnosis in the neonates is difficult because there is no definitive diagnostic test; even blood cultures have an unacceptably low sensitivity. Therefore, the clinician must accept that a number of neonates will have treatment initiated for sepsis who do not have the disease. In order to treat rapidly all infants with sepsis and to minimize therapy for those without infection, improved technology is needed for detecting the onset of sepsis and studying its long-term effect on development. This pilot project will test and idetify MRI based methods for detecting brain injuries caused by stool infection using a newly developed mouse model. Aim 1: we will quantify changes in myelination caused by sepsis using high-resolution quantitative susceptibility imaging. Aim 2: we will quantify degradations in white-matter integrity and connectivity with high-resolution diffusion tensor imaging. The project will evaluate two promissing MRI methods for the early diagnosis of white matter injuries caused by neonatal sepsis. Successful early dedection of infection allows early intervention and prevents potential damages to neural development.