TUMOR ANGIOGENESIS AND VASCULATURE MRI WITH ENDOGENOUS BOLD EFFECT

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 从根本上说，血管生成在新组织的生长和发育中起着关键作用。 然而，在肿瘤的情况下，血管生成成为快速肿瘤生长的主要促进因素。 生长和转移。 因此，血管生成抑制已经成为增加的领域。 研究人员和制药公司寻求开发新的方法， 癌症治疗传统上，量化肿瘤血管的金标准技术 微血管密度（MVD）的组织学估计。然而，这种方法是 固有的侵入性、耗时并且遭受采样偏差。克服这些 由于局限性，研究人员转向MR肿瘤血管成像。一些最成功的 所报道的技术，例如动态对比增强（DCE）MRI， 造影剂。 基于内源性对比度的方法，例如动脉自旋标记 (ASL)和高氧/高氧诱导的血氧水平依赖性（BOLD）MRI 还对挑战进行了调查。然而，这些方法不能提供足够的灵敏度 用于肿瘤脉管系统的高分辨率成像。在这项研究中，我们小心地诱导缺氧， 挑战（8%氧气吸入）以产生足够的BOLD对比度（~40%）， 能够获得高分辨率的肿瘤脉管系统图。三维高分辨率肿瘤 可以在几分钟内获得脉管系统图。该技术可以提供有效的 研究工具，我们可以量化肿瘤大小，测量肿瘤血管密度，提供早期 检测肿瘤转移，监测癌症治疗药物的有效性，并可以 潜在地表征肿瘤等级和侵袭性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Fundamentally, angiogenesis plays a key role in the growth and development of new tissues. In the case of a tumor, however, angiogenesis becomes the primary facilitator of rapid tumor growth and metastasis. As a result, angiogenesis inhibition has become an area of increased focus for researchers and pharmaceutical companies seeking to develop new methods of cancer treatment. Traditionally, the gold-standard technique to quantify tumor vasculature has been the histological estimate of microvascular density (MVD). This method, however, is inherently invasive, time consuming, and suffers from sampling bias. To overcome these limitations, researchers turned to MR tumor vasculature imaging. Some of the most successful reported techniques such as Dynamic Contrast Enhanced (DCE) MRI employ exogenous contrast agents. Methods based on the endogenous contrast, such as Arterial Spin Labeling (ASL) and Blood Oxygen Level Dependent (BOLD) MRI induced by carbogen/hyperoxic challenges were also investigated. These methods, however, do not provide sufficient sensitivity for high-resolution imaging of tumor vasculature. In this study, we carefully induced hypoxia challenge (8% oxygen inhalation) to generate sufficient BOLD contrast (~40%), with which high-resolution tumor vasculature maps were able to be obtained. 3D high-resolution tumor vasculature maps can be acquired in minutes. This technique may provide an efficient research tool for us to quantify tumor size, measure tumor vasculature density, provide early detection of tumor metastasis, monitor the effectiveness of cancer treatment drugs, and could potentially characterize tumor grade and aggressiveness.