STRUCTURE OF THE U2-U6 COMPLEX

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们正在研究S.啤酒。 这种大的RNA复合物在整个真核生物进化过程中高度保守，并形成剪接体的催化核心。 将在一定范围的温度、pH值和离子条件下研究RNA结构。 我们需要收集的NMR实验包括：1D（1H），2D NOESY，2D 1H-15 N HSQC（正常和TROSY增强），2D 1H-15 N HNN COSY，2D 1H-13 C HSQC（正常和TROSY增强），2D NOESY，2D 1H，13 C过滤/编辑NOESY。将测量各向同性和部分对齐状态下样品的残留偶极耦合（通过添加Pf 1噬菌体）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are investigating the structure of the U2-U6 snRNA complex from S. cerevisiae. This large RNA complex has been highly conserved throughout eukaryotic evolution, and forms the catalytic core of the spliceosome. The RNA structure will be investigated at a range of temperatures, pH and ionic conditions. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC (normal and TROSY enhanced), 2D 1H-15N HNN COSY, 2D 1H-13C HSQC (normal and TROSY enhanced), 2D NOESY, 2D 1H,13C filter/edit NOESY. Residual dipolar couplings will be measured for samples in both isotropic and partially aligned states (via addition of Pf1 phage).