BETA HAIRPINS OF BRAZZEIN TERMINI

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 联合突变和模拟结果表明，brazzein的末端可能是brazzein与人类甜味感受器之间的主要brazzein侧相互作用部位。Brazzein的末端参与了一个反平行的β-折叠，这使得我们能够设计来自末端的β-发夹多肽。如果末端确实是相互作用的主要部位，这些来自末端基序的多肽应该至少显示出最低的活性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Combined mutagenesis and modeling results suggest that the termini of brazzein may be the primary brazzein-side interaction site between brazzein and the human sweet taste receptor. The termini of brazzein are involved in an antiparallel beta-sheet, which has allowed us to design beta-hairpin peptides derived from the termini. If the the termini are indeed the primary site of interaction, these peptides derived from the terminal motifs should show at least minimal activity.