SWEET-RECEPTOR SATURATION TRANSFER DIFFERENCE TITRATION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 具体目标是：1.甜蛋白-受体相互作用的体外细胞测定。2.利用核磁共振技术研究甜味蛋白与甜味受体相互作用和激活甜味受体的构象和动力学要求。这些研究将有助于识别与功能特性相关的变化。3.利用STDD-NMR技术监测植物甜蛋白及其突变体与T1 R2/T1 R3甜味受体及其突变体的结合。这些结果将有助于准确定义负责brazzein-甜味受体相互作用的基本分子特征以及由此产生的信号转导无热量甜味剂作为帮助解决糖尿病和相关疾病的方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific aims are: 1. In vitro cellular assays of sweet protein-receptor interactions. 2. To use NMR spectroscopy to investigate the conformational and dynamic requirements in brazzein for its interaction and activation of the sweet taste receptor. These studies will serve to identify changes that correlate with fuctional properties. 3. To monitor binding of brazzein and its mutants to T1R2/T1R3 sweet receptor and its mutants by STDD-NMR spectroscopy. These results will contribute to accurately define the essential molecular features responsible for the brazzein-sweet receptor interaction and the resulting signal transduction non-caloric sweeteners as an approach to help addressing diabetes and related disorders.