WIFI OF ENGINEERED TISSUES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 大规模的组织工程需要具有预先发育的血管的组织。工程组织中的血管系统必须与宿主血管相连。这是如何发生的，以及如何对其进行优化，人们知之甚少。为了解决这一知识鸿沟，我们建议在具有组织工程和生物光子学专业知识的教师之间进行跨学科合作。总体目标是开发一种实时成像方法，以确定工程组织在植入活体动物后变得充满血液的时间过程。我们的中心假设是植入后48小时内会出现血流灌注。这一假设将通过追求两个具体目标来验证：1)建立一个健壮的动物模型，使宿主和植入物微血管能够实时可视化；以及2)建立宿主和植入物微血管内灌流动力学的时间进程。在第一个目标下，我们将开发一种合适的动物模型，使宿主和植入的微血管系统能够实时成像。在第二个目标下，我们将在术后长达28天的时间内测量窗腔的血流和血红蛋白血氧饱和度成像。这项拟议的研究具有创新性，因为它将建立一种新的方法来研究血管网络的数量变化以及控制这些变化的生物信号，无论是工程组织植入物还是宿主组织。这项拟议的研究意义重大，因为它将使人们能够发现一种最佳策略，以在再生医学应用中设计出具有最大临床实用价值的厚壁组织植入物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Large-scale tissue engineering requires tissues with pre-developed blood vessels. The vasculature in the engineered tissue must connect with host vessels. How this occurs and how it might be optimized are poorly understood. To address this knowledge gap, we propose an interdisciplinary collaborative effort amongst faculty with expertise in tissue engineering and biophotonics. The overall objective is to develop a real-time imaging approach to determine the time course over which the engineered tissue becomes perfused with blood after implantation in a live animal. Our central hypothesis is that perfusion will occur within 48 h after implantation. This hypothesis will be tested by pursuing two specific aims: 1) Develop a robust animal model which enables real-time visualization of host and implant microvasculature; and 2) Establish the time course of perfusion dynamics within the host and implant microvasculature. Under the first aim, we will develop a suitable animal model that enables real-time imaging of the host and implant microvasculature. Under the second aim, we will measure blood flow and hemoglobin oxygen saturation imaging of the window chamber over a period of up to 28 days post-surgery. The proposed research is innovative, because it will establish a new method to study quantitative changes in the vascular network and the biological signals which govern these changes, in both engineered tissue implants and the host tissue. The proposed research is significant, because it will enable discovery of an optimal strategy to engineer thick tissue implants with maximal clinical utility in applications of regenerative medicine.