STRUCTURES OF PROTEINS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目前正在对需要同步辐射的多种结构进行调查。其中包括多达42个蛋白质亚基的1.1和1.2个丙二醛复合体(蛋白酶体激活剂复合体)，对病毒衣壳组装和动力学至关重要的HIV-1 Gag衍生结构域的结构，在HIV发芽中起作用的细胞蛋白质和复合体，核小体重塑和重组复合体，以及血红素生物合成的酶。在许多情况下，晶体目前已准备好进行数据收集，而在其他情况下，晶体很可能在今后几个月/几年内获得。在某些情况下，我们需要同步辐射，因为衍射本身就很弱，或者因为晶体很小。其他时候，我们需要高分辨率的可靠性，查看可能指导未来药物开发努力的复合体的细节。其他时候，我们需要可调谐的辐射，以便获得相位信息或在差分图中定位反常散射中心。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Multiple structures that require synchrotron radiation are currently under investigation. These include 1.1 and 1.2 MDa complexes of up to 42 protein subunits (proteasome activator complexes), structures of HIV-1 Gag-derived domains that are important for viral capsid assembly and dynamics, cellular proteins and complexes that function in HIV budding, nucleosome remodeling and reorganizing complexes, and enzymes of heme biosynthesis. In many cases crystals are currently ready for data collection while in other cases crystals are likely to be obtained within the next few months/years. In some cases we need synchrotron radiation because the diffraction is inherently weak or because crystals are small. Other times we need high resolution to reliability see details of complexes that might guide future effort at drug development. Other times we need tunable radiation in order to obtain phase information or to localize anomalous scattering centers in difference maps.