MEASUREMENT OF GLUCOSE HOMEOSTASIS IN HUMAN BRAIN BY NMR

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 (与伊丽莎白·西奎斯特博士合作，医学，得到R01 NS035192，Pi Seaquist的支持)：该项目的总体目标是确定糖尿病的影响以及强化治疗对人类体内脑葡萄糖代谢的降糖后果。在这个资助周期中，我们用13C磁共振波谱结合13C-葡萄糖的输注来研究人脑中的糖原代谢。我们首先测定了健康志愿者在轻度和轻度高血糖时的糖原含量和周转率。然后我们研究了脑糖原在低血糖中的作用。我们假设人类大脑在低血糖期间利用糖原，并在低血糖后将其过度激活到高于正常水平(“超补偿”)，正如啮齿类动物所显示的那样。了解糖原在低血糖期间和之后在人脑中的作用非常重要，因为糖原超补偿可能涉及低血糖无意识，这是糖尿病患者胰岛素治疗的主要障碍。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. (in collaboration with Dr. Elizabeth Seaquist, Medicine, supported by R01 NS035192, PI Seaquist): The overall goal of this project is to determine the effects of diabetes and the hypoglycemic consequences of intensive therapy on in vivo brain glucose metabolism in humans. In this grant cycle, we study glycogen metabolism in the human brain by 13C MR spectroscopy in conjunction with infusions of 13C-glucose. We first determined the glycogen content and turnover rate in healthy volunteers during eu- and slight hyperglycemia. We then investigated the role of brain glycogen during hypoglycemia. We hypothesized that the human brain utilizes glycogen during hypoglycemia and overshoots it to above normal levels ("supercompensates") following hypoglycemia, as was shown to occur in rodents. Understanding the role of glycogen in the human brain during and following hypoglycemia is important because glycogen supercompensation may be involved in hypoglycemia unawareness which is a major barrier to insulin treatment in patients with diabetes.