LIPID METABOLITES AND PATHWAYS STRATEGY CONSORTIUM

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目得到了NIGMS Glue Grant的支持。整个计划有以下目标：（1）分离和检测特定细胞中的所有脂质，并发现和表征可能存在的任何新脂质。(2)定量存在的每种脂质代谢物，并定量细胞功能期间其水平和位置的变化。(3)定义每种脂质的生化途径，并开发定义相互作用网络的脂质图谱。为了帮助中性脂质核心，脂质组学重点领域，由R.C. Murphy，科罗拉多大学健康科学中心，BUSM MS资源协助确定新型中性脂质的结构。C. E.科斯特洛提供专业知识的新方法的质谱分析脂质的脂质地图计划，并出席年度会议的脂质地图调查。她在2010年5月的脂质地图研究者会议上发表了特邀演讲。与博士后研究员Hyejung Park合著的一篇文章已被接受，将在2011年5月的会议上发表。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is supported by an NIGMS Glue Grant. The overall program has the following objectives: (1) Separate and detect all of the lipids in a specific cell and discover and characterize any novel lipids that may be present. (2) Quantitate each of the lipid metabolites present and quantitate the changes in their levels and location during cellular function. (3) Define the biochemical pathways for each lipid and develop lipid maps which define the interaction networks. To aid the Neutral Lipids Core, Lipidomics Focus Area, directed by R.C. Murphy, Univ. Colorado Health Sciences Center, the BUSM MS Resource assists in the structural determinations of novel neutral lipids. C. E. Costello provides expertise on new methods for mass spectral analysis of lipids to the Lipid MAPS program and attends the annual meeting of LipidMaps investigators. She presented an invited lecture at the May 2010 meeting of Lipid Maps investigators. A posted coauthored with postdoctoral fellow Hyejung Park has been accepted for presentation at the May 2011 meeting.