Molecular Biology/Gene Expression Core

参见说明）： 分子生物学/基因表达核心设施将提供关键服务，以支持科学研究。 本计划项目的目标：1）试剂库-包含特定质粒、表达构建体、报告基因 构建体、抗体、RNA样品和逆转录病毒/腺病毒/慢病毒载体， 食管癌发生因此，这些试剂已被组织和集中。此外，本发明还提供了一种方法， 我们将继续储存新可用的试剂; 2）图像分析和基因表达定量- 可使用多种仪器，例如（但不限于）Phosphorimaging、Gel和Chemidoc、Agilent 生物分析仪和Odyssey红外蛋白质成像系统，实时定量PCR和转录 Dynex和Orion光度计分析; 3）基因微阵列基因分析服务（补贴） （Affyphon平台）通过Penn微阵列设施（鲍德温博士）和Penn生物信息学设施 (Dr. Tobias集成在生物统计学核心中）; 4）具有食管组织的人体组织库 5）食管细胞系库（鼠和人）， 原代、永生化、转化和新基因工程食管细胞系， 项目; 6）食管组织、细胞系（非转化）的食管数据库储存库（基于网络） 和转化的、器官型培养物、微阵列和共同的、共享的试剂/设备（来自Cores C和D）。分子生物学/基因表达核心提供质量保证，质量控制，成本- 其服务的有效性、及时性和效率。与人体组织相关的所有组件均为IRB 经患者知情同意书和HIPAA合规性批准，并携带患者去标识符。这一核心 与所有其他核心密切合作，特别是形态核心C，以避免重复和提供 项目的协调方法。这些项目从这个核心中受益匪浅， 相关假设和具体目标。此外，该核心在响应不断演变的P01时是动态的 需求，预测未来的P01方向，并整合新兴技术。 相关性（参见说明）：

Seeinstructions): The Molecular Biology/Gene Expression Core Facility will provide critical services to support the scientific goals of this Program Project: 1) Reagent Bank- contains specific plasmids, expression constructs, reporter constructs, antibodies, RNA samples, and retroviral/adenoviral/lentiviral vectors of relevance to the study of esophageal carcinogenesis. As a result, these reagents have been organized and centralized. In addition, we will continue to store newly available reagents; 2) Image Analysis and Gene Expression Quantification- multiple instruments are available, such as (but not limited to) Phosphorimaging, Gel and Chemidoc, Agilent Bioanalyzer and Odyssey Infrared Protein Imaging System, real-time quantitative PCR, and transcriptional analysis for Dynex and Orion Luminometers; 3) Gene Profiling Services (subsidized) with gene microarrays (Affymetrix platform) through the Penn microarray facility (Dr. Baldwin) and the Penn bioinformatics facility (Dr. Tobias integrated within the Biostatistics Core); 4) Human Tissue Bank with an esophageal tissue repository and annotated clinical tissue component; 5) Esophageal Cell Line Bank (murine and human) with primary, immortalized, transformed and newly genetically engineered esophageal cell lines for use by the Projects; 6) Esophageal database repository (web-based) for esophageal tissues, cell lines (nontransformed and transformed, organotypic cultures, microarrays, and common, shared reagents/equipment (from Cores C and D). The Molecular Biology/Gene Expression Core provides quality assurance, quality control, cost- effectiveness, timeliness and efficiency in its services. All components that pertain to human tissues are IRB approved with patient informed consent and HIPAA compliance, and carry patient de-identifiers. This Core works closely with all the other Cores, especially the Morphology Core C to avoid duplication and furnish coordinated approaches for the Projects. The Projects benefit tremendously from this Core in advancing their interrelated hypotheses and Specific Aims. In addition, this Core is dynamic in responding to evolving P01 needs, anticipating future P01 directions, and integrating emerging technologies. RELEVANCE (Seeinstructions):