The Oral Microbiome and Upper Aerodigestive Squamous Cell Cancer

描述（由申请人提供）：口腔微生物组和上呼吸消化道鳞状细胞癌我们提出了一项创新研究，以检查口腔微生物组是否与上呼吸消化道鳞状细胞癌（UADSCC：口腔，咽，喉和食管鳞状细胞癌）的风险相关。我们的研究团队已经开发出进行微生物组研究的独特能力（NIH资助：1UH2CA 140233、5 R01 AI 063477），最近报告了前肠微生物组谱与食道癌前病变的关系（Yang等人，Gastroenterology，2009），并且现在有初步数据显示口腔微生物组与口腔癌风险之间的关联。烟草和酒精是UADSCC的主要已知危险因素。我们假设口腔中的微生物可能与酒精和烟草的使用有关，从而增强UADSC的致癌作用。我们研究的目的是将口腔微生物组与UADSCC风险联系起来，并确定酒精和烟草使用对口腔微生物组的影响，这是该主题的第一次流行病学调查。我们计划在140例UADSCC病例和420例对照中，通过对口腔洗液样本中的16S rRNA微生物基因进行测序来表征所有常见的口腔微生物物种（包括不可培养物）。由于UADSCC疾病和治疗状态可以改变口腔微生物概况（反向因果关系），我们正在ACS和PLCO队列中使用前瞻性研究设计，其中在疾病发展之前收集口腔清洗样品。我们的研究将确定与UADSCC风险相关的口腔细菌谱，为实施预防性干预措施提供直接线索，作为预防UADSCC的酒精和烟草控制计划的关键步骤。 公共卫生相关性：该项目是一项创新性研究，旨在研究口腔微生物组是否与上呼吸消化道细胞癌（UADSCC：口腔，咽，喉和食管鳞状细胞癌）的风险相关。我们研究的目的是将口腔微生物组与UADSCC风险联系起来，并确定酒精和烟草使用对口腔微生物组的影响，这是该主题的第一次流行病学调查。

DESCRIPTION (provided by applicant): The Oral Microbiome and Upper Aerodigestive Squamous Cell Cancer We propose an innovative study to examine whether the oral microbiome is associated with risk of upper aerodigestive squamous cell cancers (UADSCC: oral, pharyngeal, laryngeal and esophageal squamous cell cancers). Our research team has developed a unique capability to conduct microbiome research (NIH grant: 1UH2CA140233, 5R01AI063477), have recently reported the relationship of foregut microbiome profiles with esophageal premalignant conditions (Yang et al., Gastroenterology, 2009), and now have preliminary data showing an association between the oral microbiome and oral cancer risk. Tobacco and alcohol are the major known risk factors for UADSCC. We hypothesize that microorganisms in the oral cavity potentiate UADSC carcinogenesis, potentially related to alcohol and tobacco use. The goals of our study are to relate the oral microbiome to UADSCC risk and to identify the impact of alcohol and tobacco use on the oral microbiome, in the first epidemiologic investigation of this topic. We plan to characterize, in 140 UADSCC cases and 420 controls, all common oral microbial species (including non-culturables) by sequencing the 16S rRNA microbial genes in oral wash samples. Because UADSCC disease and treatment status can alter oral microbial profiles (reverse causation), we are using a prospective research design, in the ACS and PLCO cohorts in which oral wash samples were collected prior to disease development. Our study will identifying oral bacterial profiles related to UADSCC risk, providing direct leads to implement prophylactic interventions as critical adjuncts to alcohol and tobacco control programs in UADSCC prevention. PUBLIC HEALTH RELEVANCE: The proposed project is an innovative study to examine whether the oral microbiome is associated with risk of upper aerodigestive cell cancers (UADSCC: oral, pharyngeal, laryngeal and esophageal squamous cell cancers). The goals of our study are to relate the oral microbiome to UADSCC risk and to identify the impact of alcohol and tobacco use on the oral microbiome, in the first epidemiologic investigation of this topic.