Immunology Core

这个共享资源核心的总体目标是提供全面的免疫学支持 加强SE STI CRC调查员的活动。该核心将提供监测方面的专业知识 系统和粘膜免疫，重点是体液、细胞和生物标记物分析。《核心》 将利用体内免疫策略、体外再刺激试验、最先进的免疫表型 以及流式细胞术，体液反应的酶联免疫吸附试验和亲和力(SPR)分析，以及多种炎症反应 生物标志物分析。为了实现核心目标，塞姆波斯基博士的细胞 免疫学实验室和斯塔茨博士的粘膜免疫学实验室已经合并。核心领导者之外 向他们提供以下杜克人类疫苗研究所共享资源：研究流程 细胞学、蛋白质组学-生物核心和免疫重建。和生物标记物分析。这些共享 资源拥有所需的最先进的工具，可以为 SE STI CRC。将免疫学核心纳入SE STI CRC将提供一套通用的 为SE STI CRC调查人员提供的宿主免疫反应机械分析平台。In的用法 体外再刺激试验、流式细胞术和ELISA/SPR将允许全面的宿主反应 对挑战病原体和实验性疫苗/佐剂进行监测。血液、组织和培养 上清液炎症生物标志物分析将进一步提供对特定宿主反应的有价值的洞察 将加强中心项目提出的挑战和机制研究的途径，以及 协助确定SE STI CRC制定的疫苗接种方案的免疫相关因素。 这一免疫学核心支持的积极的性传播感染研究领域包括基础先天和 淋病奈瑟菌的获得性免疫反应(系统免疫和粘膜免疫)及其鉴定和发展 新型佐剂增强对淋球菌保护性免疫的诱导

The overall objective of this Shared Resource Core is to provide comprehensive Immunology support to enhance the activities of the SE STI CRC investigators. The core will provide expertise in monitoring systemic and mucosal immunity with an emphasis on humoral, cellular and biomarker analyses. The Core will utilize in vivo immunization strategies, in vitro restimulation assays, state-of-the-art immunophenotyping and flow cytometry, ELISA and avidity (SPR) analysis of humoral responses, and multiplex inflammatory biomarker analysis. To accomplish Core goals the efforts and expertise of Dr. Sempowski's cellular immunology lab and Dr. Staats' mucosal immunology lab have been combined. Core leaders in addition have available to them the following Duke Human Vaccine Institute Shared Resources: Research Flow Cytometry, Proteomics-Biacore, and Immune Reconstitution. and Biomarker Analysis. These shared resources have the state-of-the-art instrumentation needed to provide worid-class immune monitoring to the SE STI CRC. The inclusion on an Immunology Core in the SE STI CRC will provide a common set of platforms for mechanistic analysis of host immune response for the SE STI CRC investigators. The use of in vitro restimulation assays, flow cytometry and ELISA/SPR will allow comprehensive host response monitoring to both challenge pathogens and experimental vaccines/adjuvants. Blood, tissue and culture supernatant inflammatory biomarker analysis will provide further valuable insight into specific host response pathways which will enhance the challenge and mechanistic studies proposed by center projects, as well as assist in identification of correlates of immunity for vaccination regimens developed by the SE STI CRC. Areas of active STI investigation to be supported by this Immunology Core include fundamental innate and adaptive immune responses (systemic and mucosal) to N. gonorrhoeae, and identification and development of novel adjuvants to augment induction of protective immunity against N. gonorrhoeae