Neuropathology and Tissue Culture Core

核心A：神经病理学和细胞培养核心 神经病理学和细胞培养核心是该计划项目资助（PPG）的科学核心。的 该核心的核心作用是协助PPG内三个研究项目的总体目标， 确定艾滋病患者中HIV脑炎发展过程中涉及的不同分子途径。 神经病理学和细胞培养核心将通过提供以下服务来帮助计划项目的参与者： 来自诊断为HIV脑炎的HIV-1感染患者的手术和尸检脑组织材料， 以及来自没有CNS受累的HIV-1感染个体的脑和正常对照脑。密切 与项目1，2和3合作，我们将进行免疫组织化学分析，包括双 病毒蛋白、细胞标志物和Rho/Rac途径的不同下游分子的标记 以及它们与Pur-a（项目1）、TNF α和Ras通路及其与IGF-1的关联。 1/IRS途径（项目2）和HIF-1途径及其与Vpr的关联（项目3）。的 神经病理学和细胞培养中心还将提供高度纯化的人类胎儿小胶质细胞培养物， 星形胶质细胞和神经元。将从大鼠和小鼠中制备神经元的原代培养物 海马区进行研究。建立的细胞系，如PC 12，将被维持、培养， 分配到项目。此外，将制备来自HIV-1感染细胞的条件培养基以供实验室使用。 个别项目。最后，核心将提供分化成不同细胞的神经球培养物， 表型，包括神经元、少突胶质细胞和星形胶质细胞。拟议的实验将有助于项目 1，2和3，以破译参与HIV脑炎发病机制的分子途径。

Core A: The Neuropathology and Cell Culture Core The Neuropathology and Cell Culture Core is the scientific core of this Program Project Grant (PPG). The central role of this Core is to assist the overall objectives of the three research projects within the PPG to determine the different molecular pathways involved in the development of HIV encephalitis in AIDS patients. The Neuropathology and Cell Culture Core will assist the participants of the Program Project by providing surgical and autopsy brain tissue material from HIV-1 infected patients diagnosed with HIV encephalitis, as well as brains from HIV-1 infected individuals with no CNS involvement and normal control brains. In close collaboration with Projects 1, 2 and 3, we will perform immunohistochemical analysis, including double labeling, of viral proteins, cellular markers, and the different downstream molecules of the Rho/Rac pathway and their association with Pur-a (Project 1), the TNFa and Ras pathway and its association with the IGF- 1/IRS pathway (Project 2), and the HIF-1 pathway and its association with Vpr (Project 3). The Neuropathology and Cell Culture Core will also provide highly purified cultures of human fetal microglia, astrocytes, and neurons on demand. Primary cultures of neurons will be prepared from rat and mouse hippocampus for the proposed studies. Established cell lines, such as PC12 will be maintained, cultured, and distributed to the projects. Also, Conditioned Media from HIV-1 infected cells will be prepared for use by the individual projects. Finally, the Core will provide cultures of neurospheres differentiated into different cellular phenotypes, including neurons, oligodendrocytes and astrocytes. The proposed experiments will aid Projects 1, 2 and 3 to decipher the molecular pathways involved in the pathogenesis of HIV encephalitis.