MICROSCOPY CORE

NMS核心： 1.计算机控制的电动直立荧光显微镜（Zeiss Axioimager），具有压电控制的载物台和微扫描CCD相机。该显微镜配备了良好的阵列断层扫描数据采集。 2.具有光谱成像能力的倒置激光扫描共聚焦显微镜（Zeiss LSM 510 Meta）和用于活细胞延时成像的环境控制室。 3.两个直立的双光子显微镜在一个大型光学工作台上，共享一对钛宝石飞秒脉冲激光器。这两种显微镜都配备了同时双光子成像和双光子光活化。可配置的工作台光学器件还允许在双波长下同时成像。a.一个显微镜被设计用于体内成像（Ultima IV，Prairie Technologies，Middleton，WI）。 B.另一个显微镜被设计用于组织切片成像（Ultima XY，Prairie Technologies，Inc.

NMS Core: 1. A computer-controlled, motorized upright fluorescence microscope (Zeiss Axioimager), with piezo-controlled stage and microscanning CCD camera. This microscope is well-equipped for Array Tomography data acquisition. 2. An inverted, laser scanning confocal microscope with spectral imaging capability (Zeiss LSM510 Meta) and an environmental control chamber for time-lapse imaging of live cells. 3. Two upright two-photon microscopes on a single large optical table, sharing a pair of Ti:sapphire femtosecond pulsed lasers. Both microscopes are equipped for simultaneous two-photon imaging and twophoton photoactivation. Configurable table optics also allow for simultaenous imaging at dual wavelengths. a. One microscope is designed for in vivo imaging (Ultima IV, Prairie Technologies, Middleton, Wl). b. The other microscope is designed for tissue slice imaging (Ultima XY, Prairie Technologi