Experience Dependent Plasticity in a fosGFP Mouse

描述（由申请人提供）：控制突触超可塑性（可塑性的可塑性）的规则在发育中的神经系统中特别重要，其中经验在神经反应的模式中发挥着重要作用，并且先前突触活动对未来突触的影响变化可能会产生深远的后果。我们已经开发并采用了fosGFP转基因小鼠，以确定在体感新皮层的经验依赖性变化的轨迹。在对照动物中，NMDAR依赖性LTP可以在体外第4-2/3层突触产生，并且正常经验导致这些突触在出生后第二周和第三周逐渐加强。胡须刺激增强突触强度，并导致可塑性的分子机制从NMDAR依赖性增强转变为mGluR依赖性增强。该提案旨在确定如何累积的，经验依赖性的可塑性启动和维持激活特定亚型的谷氨酸受体，使用在体内和体外分析。

DESCRIPTION (provided by applicant): The rules governing synaptic metaplasticity (the plasticity of plasticity) are of special importance in the developing nervous system, where experience has an important role in the patterning of neural responses and the influence of prior synaptic activity on future synaptic change can have profound consequences. We have developed and employed a fosGFP transgenic mouse to identify the locus of experience-dependent change in somatosensory neocortex. In control animals, NMDAR-dependent LTP can be generated at layer 4-2/3 synapses in vitro, and normal experience results in the progressive strengthening of these synapses over the second and third postnatal week. Whisker stimulation enhances synaptic strength and leads to a transformation in the molecular mechanisms that underlie plasticity, from NMDAR-dependent to mGluR-dependent potentiation. This proposal seeks to identify how cumulative, experience-dependent plasticity is initiated and maintained by the activation by specific subtypes of glutamate receptors, using both in vivo and in vitro analysis.