TRANSGENIC MOUSE AND EMBRYONIC STEM CELL FACILITY

芝加哥大学综合癌症中心（UCCCC）转基因小鼠/胚胎干细胞设施提供全面的基因工程服务，以改变实验室小鼠的基因组。UCCCC有许多使用小鼠模型作为主要分析工具的研究人员，以及许多在研究癌症的原因和治疗时偶尔需要小鼠模型的额外研究人员。通过显微注射单细胞小鼠胚胎产生和维持转基因动物，以及通过使用ES细胞产生转基因小鼠，都需要专门的技术人员。此外，这种努力需要购置一系列专用设备。因此，共享资源的可用性极大地降低了UCCCC个人研究者的研究成本。该设施的存在也大大增加了相关经验有限的调查人员获得遗传工程技术的机会。 UCCCC转基因小鼠/胚胎干细胞设施成立于1991年，并取得了巨大的成效和成功;自成立以来，所提供的服务范围已大大扩大。转基因小鼠/胚胎干细胞设施提供的服务包括： 1)转基因小鼠从创始人到F1阶段的生产; 2）ES细胞技术小鼠 生产; 3）ES细胞基因靶向和培养; 4）胚胎再衍生; 5）小鼠胚胎饲养（MEF）细胞生产; 6）各种品系和品系小鼠的定时妊娠; 7）各种育种服务和GEM模型系维持; 8）从ES细胞系制备DNA;和9）转基因或ES细胞靶向载体的设计和构建。除了技术服务外，该基金还协助设计需要小鼠分子遗传学的研究，并就小鼠的处理和繁殖提供咨询和指导。在提供这些综合服务的过程中，UCCCC转基因小鼠/胚胎干细胞设施已经产生了小鼠模型， 导致我们对癌症的理解的进步，包括乳腺癌，皮肤癌，结肠癌，脑癌和前列腺癌。

The University of Chicago Comprehensive Cancer Center (UCCCC) Transgenic Mouse/Embryonic Stem Cell Facility provides comprehensive genetic engineering services to alter the genome of the laboratory mouse. The UCCCC has many invesfigators who use mouse models as their primary tool of analysis, as well as a multitude of addifional investigators who require the occasional mouse model in their studies of the causes and treatment of cancer. The generation and maintenance of transgenic animals through the microinjection of single-celled mouse embryos, and the generation of genetically modified mice through the use of ES cells require specialized technical personnel. Furthermore, such efforts necessitate the acquisition of an array of devoted equipment. Thus, the availability of a shared resource greatly reduces research costs for the individual investigators of the UCCCC. The existence of this Facility also greatly increases the accessibility of genetic engineering technology to investigators with limited related experience. The UCCCC Transgenic Mouse/Embryonic Stem Cell Facility was established in 1991, and has been tremendously productive and successful; the range of services provided has expanded considerably since it's founding. Services provided by the Transgenic Mouse/Embryonic Stem Cell Facility include: 1) transgenic mouse production from founder through F1 Stage; 2) ES cell technology mouse production; 3) ES cell gene targeting and culturing; 4) embryo rederivation; 5) mouse embryonic feeder (MEF) cell production; 6) Timed pregnancies of various strains and lines of mice; 7); various breeding services and GEM model line maintenance; 8) DNA preparation from ES cell lines; and 9) design and construction of transgenic or ES cell targeting vectors. In addition to the technical services, the Facility also offers assistance with the design of studies that require mouse molecular genetics, as well as advice and instruction on mouse handling and breeding. In providing these comprehensive services, the UCCCC Transgenic Mouse/Embryonic Stem Cell Facility has generated mouse models that have led to advances in our understanding of cancer, including cancers of the breast, skin, colon, brain and prostate.