Metabolism Core

新陈代谢核心的设计是为了在清醒的小鼠身上进行体内和生理实验 转基因小鼠模型的详细代谢表型有助于理解肥胖的目的， 糖尿病及其并发症。本中心在DAE总监Jason Kim博士的监督下运作 Young Jung(联席主任)和Hwi jin Ko(联席主任)，负责监督核心和 与UMassMMPC的用户互动。核心的主要职能是执行以下任务 实验：1)高胰岛素-正常血糖钳夹评估器官特异性胰岛素作用和血糖 清醒小鼠的代谢，2)高血糖钳夹评估体内胰岛β细胞功能 清醒小鼠，3)静脉或ip葡萄糖/胰岛素耐量试验，4)颈静脉插管存活手术 活体实验，5)实施渗透泵用于药物或其他药物的慢性给药，6) 特殊饮食的应用(例如。高脂饮食)，以改变能量平衡并诱导小鼠肥胖。7)急性 脂肪输注、细胞因子和根茎苷急/慢性给药，以及STZ注射剂改变血糖 动态平衡。8)无创测量能量消耗、呼吸交换率、体力活动 使用代谢笼的清醒小鼠的活动量和食物/水摄入量。9)非侵入性评估 用1H-MRS对清醒小鼠的全身、器官和组织的脂肪/瘦肉/水团进行活检。 10)实施运动研究笼轮，12)细胞内代谢生化分析 流量测量(糖酵解、糖原合成)。新陈代谢核心有规律地与 用于补充、高通量分析分析的分析核心，用于测量激素、细胞因子和 代谢物使用在代谢实验结束时获得的血清样本。还有，新陈代谢的核心 与心血管并发症核心相互作用并与其相关的其他表型 心血管功能。新陈代谢核心的目标是使用一系列优雅的、非侵入性的和 活体实验，从单个小鼠身上获得一组强大的代谢数据，以便提供 对选定基因在肥胖、胰岛素抵抗和糖尿病中的作用的机械性见解。

The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the purpose of detailed metabolic phenotyping of transgenic mouse models useful for understanding obesity, diabetes and its complications. The Core operates under the supervision of Drs. Jason Kim (Director), Dae Young Jung (co-Director), and Hwi Jin Ko (co-Director) who oversee day-to-day operation of the Core and interact with the users of the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1) hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious mice, 2) hyperglycemic clamp to assess in vivo pancreatic beta-cell function in conscious mice, 3) iv or ip glucose/insulin tolerance tests, 4) survival surgery of jugular vein cannulation for in vivo experiments, 5) implementation of osmotic pumps for chronic delivery of drugs or other agents, 6) application of special diet (e.g.. high-fat diet) to alter energy balance and induce obesity in mice. 7) acute lipid infusion, acute/chronic delivery of cytokines and phloridzin, and STZ injection to alter glucose homeostasis. 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical activity, and food/water intake in conscious mice using metabolic cages. 9) non-invasive assessment of whole body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1 H-MRS. 10) implementation of cage wheel for exercise study, and 12) biochemical analysis for intracellular metabolic flux measurement (glycolysis, glycogen synthesis). The Metabolism Core regulariy interacts with the Analytical Core for complementary, high-throughput analytical assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic experiments. Also, the Metabolism Core interacts and with the Cardiovascular Complications Core for additional phenotyping associated with cardiovascular functions. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the role of selected gene on obesity, insulin resistance, and diabetes.