15 Tesla Bruker SolariX FT-ICR mass spectrometer

描述(由申请者提供)：我们申请资金购买一台15T Bruker Daltonics SolariX FT-ICR质谱仪，具有双ESI/MALDI源。该仪器将为NIH资助的用户提供关键需求，包括高质量分辨率、高质量精度和高通量数据采集；增强的灵敏度和动态范围；高空间分辨率的高性能分子成像；以及与我们目前的FTMS系统和其他商业化的高性能质量分析仪相比，质量范围更广。购买的仪器将取代我们目前的9.4T Apex-QE FT-ICR MS。选择15T SolariX系统的主要驱动因素是较高磁场的动态范围显著增加，以及仪器组件的整体性能提高，包括执行高速CASI实验的能力，这项技术可以将选定质量窗口的动态范围提高100倍以上。该仪器将支持不同的主要和次要用户群体，以及由国家卫生研究院资助的范德比尔特大学研究界作为一个整体。美国国立卫生研究院资助的主要用户如下。Kevin Schey博士：水通道蛋白-0在晶状体发育和老化中的作用特伦斯·S·德莫迪博士：了解呼肠孤病毒诱导中枢神经系统细胞凋亡的细胞机制。克利夫顿·E·巴里博士，III：结核分枝杆菌独特的病变类型和化疗反应。比利·G·哈德森博士：糖尿病肾病中基底膜和肾小球中蛋白质的葡萄糖修饰。H·斯科特·鲍德温博士：半月瓣向儿科组织工程瓣膜成熟的蛋白质组学特征。Volker Hans Haase博士：了解HIF-1和HIF-2在肾损伤进展中的作用。15T Bruker SolariX FT-ICR仪器将安装在范德比尔特大学医学院的质谱学研究中心。该仪器将由6名专业人员组成的团队管理，其中包括一名FT-ICR质谱学专家和一名质谱学工程师。业务资金将来自向调查人员评估的使用费，用于使用MSRC服务核心多年来一直使用的标准会计和计费程序进行服务。设立了咨询委员会，以确保拟议文书的适当使用和可持续性。拟议的仪器将为Vanderbilt社区带来关键和无与伦比的新能力，为高度复杂的生物样本提供高度准确、敏感和详细的信息。 与公众健康相关：所要求的15T Bruker Daltonics SolariX FT-ICR质谱仪提供关键的、无与伦比的新功能，提供高质量精度、高灵敏度和更大的动态范围，以便为范德比尔特大学社区提供高度复杂的生物样本的详细信息。这种高性能仪器是提供新信息以帮助理解生物系统分子复杂性的必要工具。

DESCRIPTION (provided by applicant): We request funds for a 15T Bruker Daltonics solariX FT-ICR mass spectrometer with a dual ESI/MALDI source. The instrument will provide NIH-funded users with critical needs including high mass resolving power, high mass accuracy and high-throughput data acquisition; enhanced sensitivity and dynamic range; high- spatial resolution high-performance molecular imaging; and an expanded mass range in comparison to our current FTMS system and other commercially available high-performance mass analyzers. The purchased instrument will replace our current 9.4T Apex-Qe FT-ICR MS. Major drivers for the choice the 15T solariX system is the significantly increased dynamic range from the higher magnetic field and the overall increase in performance from the instrumental components including the ability to perform high-speed CASI experiments, a technique that can enhance the dynamic range of selected mass windows over 100-fold. The instrument will support a diverse group of major and minor users and the extensively NIH-funded Vanderbilt University research community as a whole. Highlighted NIH funded Major Users are as follows. Dr. Kevin Schey: The Role of Aquaporin-0 in Lens Development and Aging. Dr. Terence S. Dermody: Understanding Cellular Mechanisms Mediating Reovirus-Induced Apoptosis in the Central Nervous System. Dr. Clifton E. Barry, III: Unique Lesion Types and Chemotherapy Response of M. tuberculosis. Dr. Billy G. Hudson: Modification of Proteins in the Basement Membrane and Glomerulus by Glucose in Diabetic Nephropathy. Dr. H. Scott Baldwin: Proteomic Characterization of Semilunar Valve Maturation towards Pediatric Tissue Engineered Valves. Dr. Volker Hans Haase: Understanding the Role of HIF-1 and HIF-2 in the Progression of Renal Injury. The 15T Bruker solariX FT-ICR instrument will be located in the Mass Spectrometry Research Center at Vanderbilt University School of Medicine. The instrument will be managed by a team of 6 professionals including a FT-ICR mass spectrometry specialist and a mass spectrometry engineer. Operational funds will be generated from user fees assessed to investigators for service using standard accounting and billing procedures that have been used by the MSRC services cores for years. Advisory committees are in place to ensure proper use and sustainability of the proposed instrument. The proposed instrument will bring critical and unparalleled new capabilities to the Vanderbilt community providing highly accurate, sensitive and detailed information for highly complex biological samples. PUBLIC HEALTH RELEVANCE: The requested 15T Bruker Daltonics solariX FT-ICR mass spectrometer delivers critical and unparalleled new capabilities in providing high mass accuracy, high sensitivity and much greater dynamic range so as to provide detailed information for highly complex biological samples for the Vanderbilt University community. This high performance instrument is a necessary tool to provide new information to help understand the molecular complexity of biological systems.