Establishment & maintenance of a closed CPRC SPF colony

描述（由申请人提供）：本提案的目的是在加勒比灵长类动物研究中心（CPRC）的Sabana Seca野外站维持一个封闭的无特定病原体（SPF）恒河猴供应和繁殖群计划。由CPRC捐赠的具有遗传特征的印度来源猴的原始种群将包括约755只SPF（无疱疹B、STLV-1、SIV和SRV）恒河猴，其中164只（或22%）也将不含乳头状病毒SV 40。因此，该菌落将由两个SPF亚菌落组成：一个无SV 40，另一个SV 40阳性。将来自这两个封闭SPF亚群的动物饲养在繁殖笼中，并每季度检测是否存在上述靶病毒。一组高度多态性的遗传标记将用于表征殖民地并优化育种群体的结构，最大限度地提高其遗传异质性水平。到第五年，该计划将产生总共1,082只SPF恒河猴的后代，其中301只也将是SV 40阴性。这些SPF猕猴将有助于满足对这些动物日益增长的研究需求。在这5年的补助金结束时，预计该计划将产生超过160只动物/年，并将实现自给自足。CPRC将继续为艾滋病和其他研究项目做出重大贡献，为NIH赞助的研究项目提供高质量和健康的SPF恒河猴。

DESCRIPTION (provided by applicant): The objective of this proposal is to maintain a closed specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically-characterized Indian-origin monkeys donated by CPRC will consist of approximately 755 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques of which 164 (or 22%) will also be free of the papova virus SV40. Thus, this colony will consist of two SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these two closed SPF sub colonies will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 1,082 SPF rhesus macaques, of which 301 will be also SV40 negative. These SPF macaques will help meet the increased research demand for these animals. At the end of this 5 year grant, it is expected that this program will produce more than 160 animals/year and will attain self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.