Project 2: Mid-Gestational Exposure to EDCs and Effects on Placental Developmen

具体目标：研究项目2测试了以下假设：多溴二苯醚(PBDEs)、全氟化化学品(PFCs)和环境有机酸(EOAs)在人类胎儿和怀孕中期的积累不同，这些化学物质暴露对胎盘发育和功能产生负面影响，这是对子宫生长和发育产生不利影响的重要后果。因此，目标是通过传统和新的生物监测方法(目标1)比较妊娠中期孕妇血清、胎盘和胎儿肝脏中多溴二苯醚、全氟碳化合物和部分EOAs的水平(目标1)；并通过分析滋养细胞分化和功能的形态和分子标记物来评估这些化学物质暴露与胎盘发育中断之间的关系(目标2)。

Specific Aims: Research Project 2 tests the hypotheses that polybrominated diphenyl ethers (PBDEs), perfluorinated chemicals (PFCs) and environmental organic acids (EOAs) accumulate differently in human fetuses than in pregnant women during mid-gestation, and that these chemical exposures negatively impact placental development and function, important outcomes that can adversely affect in utero growth and development. Accordingly, the goal is to compare 2nd trimester maternal serum, placenta and fetal liver levels of PBDEs, PFCs and select EOAs through traditional and novel biomonitoring approaches (Aim 1); and evaluate the relationship between these chemical exposures and disruptions of placental development by analyzing morphological and molecular markers of trophoblast differentiation and function (Aim 2).