Mouse Genetics Core Facility

转基因小鼠提供了一种易于处理的方法来测试蛋白质功能在血栓形成和白细胞渗出等复杂过程中的生物学意义。CORE A将作为小鼠遗传学的核心设施，在小鼠基因打靶和转基因项目的设计和实施中为项目研究人员提供建议、培训和服务，包括ES细胞培养、同源重组的转基因和筛选、囊胚注射，以及方正品系中生殖系传播等位基因的特征。此外，核心A将为项目研究人员提供进入核心维护的小鼠资料库的途径，其中包括血管细胞(血小板、内皮细胞、平滑肌细胞和几种类型的白细胞)中有条件删除或过度表达蛋白质的Cre表达系。

Genetically-modified mice provide a tractable approach to test the biological significance of protein function in complex processes such as thrombosis and leukocyte extravasation. Core A will serve as a mouse genetics core facility to provide advice, training, and services to project investigators in the design and implementation of mouse gene targeting and transgenesis projects including ES cell culture, transfection and screening for homologous recombination, blastocyst injection, and characterization of germline transmitted alleles in founder lines. In addition, Core A will provide project investigators with access to a library of mice maintained by the Core including Cre expressing lines for conditional deletion or overexpression of proteins in vascular cells (platelets, endothelial cells, smooth muscle cells, several types of leukocytes).