Metabolism Core

代谢核心设计用于在清醒小鼠中进行体内和生理实验， 转基因小鼠模型的详细代谢表型分析的目的， 糖尿病及其并发症。核心在Jason Kim博士（主任）、Dae Young Jung（联席董事）和Hwi Jin Ko（联席董事）负责核心和 与麻省大学MMPC的用户互动。核心的主要职能是执行以下工作 实验：1）高胰岛素-正常血糖钳夹以评估器官特异性胰岛素作用和葡萄糖 2）高血糖钳夹以评估在清醒小鼠中的体内胰腺β细胞功能， 清醒小鼠，3）静脉或腹腔注射葡萄糖/胰岛素耐量试验，4）颈静脉插管的存活手术， 体内实验，5）用于长期递送药物或其它药剂的渗透泵的实施，6） 特殊饮食的应用（例如，高脂肪饮食）以改变小鼠的能量平衡并诱导肥胖。7)急性 脂质输注、细胞因子和根皮苷的急性/慢性递送以及STZ注射以改变葡萄糖 体内平衡8)能量消耗的无创测量，呼吸交换率，物理 活动和使用代谢笼的清醒小鼠的食物/水摄入量。9)非侵入性评估 使用1H-MRS在清醒小鼠中的脂肪/瘦/水质量的全身、器官和组织活检组成。 10)用于运动研究笼轮的实施，和12）用于细胞内代谢的生化分析 通量测量（糖酵解、糖原合成）。代谢核心定期与 用于测量激素、细胞因子和细胞因子的互补、高通量分析测定的分析核心 使用代谢实验结束时获得的血清样品测定代谢物。此外，新陈代谢核心 与心血管并发症核心相互作用，以获得与 心血管功能代谢核心的目标是使用一系列优雅的，非侵入性的， 体内实验以从个体小鼠获得稳健的代谢数据集， 对选定基因在肥胖、胰岛素抵抗和糖尿病中的作用的机制性见解。

The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the purpose of detailed metabolic phenotyping of transgenic mouse models useful for understanding obesity, diabetes and its complications. The Core operates under the supervision of Drs. Jason Kim (Director), Dae Young Jung (co-Director), and Hwi Jin Ko (co-Director) who oversee day-to-day operation of the Core and interact with the users of the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1) hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious mice, 2) hyperglycemic clamp to assess in vivo pancreatic beta-cell function in conscious mice, 3) iv or ip glucose/insulin tolerance tests, 4) survival surgery of jugular vein cannulation for in vivo experiments, 5) implementation of osmotic pumps for chronic delivery of drugs or other agents, 6) application of special diet (e.g.. high-fat diet) to alter energy balance and induce obesity in mice. 7) acute lipid infusion, acute/chronic delivery of cytokines and phloridzin, and STZ injection to alter glucose homeostasis. 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical activity, and food/water intake in conscious mice using metabolic cages. 9) non-invasive assessment of whole body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1 H-MRS. 10) implementation of cage wheel for exercise study, and 12) biochemical analysis for intracellular metabolic flux measurement (glycolysis, glycogen synthesis). The Metabolism Core regulariy interacts with the Analytical Core for complementary, high-throughput analytical assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic experiments. Also, the Metabolism Core interacts and with the Cardiovascular Complications Core for additional phenotyping associated with cardiovascular functions. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the role of selected gene on obesity, insulin resistance, and diabetes.