Gene Transfer Core, NIDCR-DIR

在上一财年，GTC生成了3个基因敲除（KO）小鼠模型。自GTC成立以来，共生成了71个KO小鼠模型。在2014财年共产生了151个转基因小鼠品系，使该服务自开始以来的总数达到2147个小鼠品系。在FY13至FY14，GTC引入了新型基因编辑构建体的显微注射，如锌指核酸酶（ZFN），转录激活因子样效应核酸酶（TALENS）和最近的规则间隔短回文重复序列（CRISPR）。GTC在2014财年将49只小鼠模型作为精子冷冻保存，其中28只小鼠模型在卫星冷冻机（Porter Building）中冷冻保存。用冷冻精子和胚胎成功地再衍生出17个小鼠模型。GTC在2014财年从12个小鼠模型中纯化了基因组DNA，并使用PCR鉴定了正确的基因型。正如ZIA的其他报告所示，2014财年发表了许多基于GTC生成的小鼠模型的期刊文章。

In the previous fiscal year, the GTC generated 3 knockout (KO) mouse models. Since the inception of the GTC, a total of 71 KO mouse models have been generated. A total of 151 transgenic mouse lines were generated in FY14, bringing the total since inception of this service to 2147 mouse lines. In FY13 through FY14, the GTC introduced microinjection of novel gene-editing constructs such as Zinc Finger Nucleases (ZFN), Transcription Activator-like Effector Nucleases (TALENS) and most recently, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). The GTC cryopreserved 49 mouse models as sperm in FY14, of which 28 mouse models were cryopreserved in a satellite freezer (Porter Building). 17 mouse models were successfully rederived from frozen sperm and embryos. GTC purified genomic DNA from 12 mouse models in FY14 and used PCR to identify the correct genotype.As indicated in others ZIA reports, numerous journal articles were published in FY14 based on the mouse models generated by the GTC.