CELL AND MOLECULAR IMAGING

细胞和分子成像（CMI）共享资源的使命是为霍林斯癌症中心（HCC）的成员提供一套完全支持的最先进的成像能力。这些能力包括在细胞、组织、活体和体内整个动物水平的成像。在第一个CCSG项目期间，为了满足HCC研究人员日益增长的需求，能力和服务得到了显著扩展，在主任John J. Lemasters博士和副主任Anna-Liisa Nieminen博士和Ann-玛丽布鲁姆博士的领导下，现已由两个协同和协调的单位组成-共聚焦和多光子显微镜单元和小动物成像单元在一个共享资源中整合这些成像方法是为了认识到新兴技术的依赖以及研究者前瞻性地考虑其成像策略以有效地将其研究从早期的细胞和组织研究到具有临床前影响的整个动物应用。自2009年以来，CMI共享资源支持了34名研究人员代表所有HCC研究项目和7个MUSC部门的研究，导致超过71篇出版物。 HCC CMI共享资源的具体目的是提供： ▪获得最先进的共聚焦和多光子显微镜，用于培养中的活细胞成像、活体动物活体成像和体内小动物成像，包括MRI、fMRI、MR光谱学、microCT、microPET、生物发光、荧光和X射线。 在多种成像模式，包括显微镜技术，活细胞成像， 多光子成像和临床前体内动物成像。 提供有关实验设计、样品制备、探针选择、成像应用数据分析以及将显微镜评估转换为整体动物成像的咨询和协助。 在成像技术及其应用的基础教育，这是必要的适当利用这些方法，并传播有关新的实验策略，新兴技术和数据分析技术的持续发展的信息。

The mission of the Cell & Molecular Imaging (CMI) Shared Resource is to provide members of the Hollings Cancer Center (HCC) with a fully supported suite of state-of-the-art imaging capabilities. These capabilities include imaging at the cellular, tissue, intravital, and in vivo whole animal levels. Based on significant , expansion of capabilities and services to meet the growing needs of HCC investigators during the first CCSG project period, this resource, led by Dr. John J. Lemasters, Director, and Drs. Anna-Liisa Nieminen and Ann- Marie Broome, Associate Directors, has evolved significantly and now consists of two synergistic and coordinated units - the Confocal & Multiphoton Microscopy Unit and the Small Animal Imaging Unit The integration of these imaging approaches within one shared resource has been undertaken in recognition of the inter-reliance of the emerging techniques as well as the need for investigators to prospectively consider their imaging strategies to effectively transition their research from early cell and tissue studies to whole animal applications with preclinical impact. Since 2009, the CMI Shared Resource has supported research leading to more than 71 publications by 34 investigators representing all HCC research programs and seven MUSC departments. The specific purposes of the HCC CMI Shared Resource are to provide: ▪ Access to state-of-the-art confocal and multiphoton microscopy for imaging living cells in culture, intravital imaging in live animals, and in vivo small animal imaging, including MRI, fMRI, MR spectroscopy, microCT, microPET, bioluminescence, fluorescence, and X-ray. ▪ In depth training in multiple imaging modalities, including microscopic techniques, live cell imaging, multiphoton imaging, and pre-clinical in vivo animal imaging. ▪ Consultation and assistance concerning experimental design, sample preparation, probe selection, data analysis for imaging applications, and transitioning microscopic evaluations to whole animal imaging. Education in the fundamentals of imaging technology and its application, which are essential for appropriate utilization of these approaches, and dissemination of information concerning the ongoing development of novel experimental strategies, emerging technologies, and data analysis techniques.