Examining novel roles of nicotine and tobacco smoke on RNA biology

我们的假设是尼古丁和/或传统烟草烟雾暴露会通过诱导替代AP模式和异构体形成来改变RNA生物学。为了调查这两种假设，我们目前正在进行初步的试点研究，使用正常和癌症细胞系，接触和不接触尼古丁和烟草烟雾冷凝物。我们正在进行一种新的3 prime utr特异性rna测序方法来检查APA模式。根据这些分析的结果，我们随后将对NCI-MD病例对照研究中的人类肺癌和正常组织进行分析，以在人类肺癌中提出这个问题。为了研究这一假设的第二部分，我们正在执行类似的方案，但我们对样本进行了miRNA测序。isomiR形成的表达差异将在后续研究中进一步表征其功能意义。

Our hypothesis is that nicotine and/or traditional tobacco smoke exposure will modify RNA biology via inducing alternative AP patterns and isomiR formation. To investigate these two hypotheses, we are now conducting preliminary pilot studies using normal and cancer cell lines, with and without nicotine and tobacco smoke condensate exposure. We are conducting a novel 3 prime UTR-specific RNA-sequencing method to examine APA patterns. Depending on the results of these analyses, we will subsequently conduct an analysis of human lung cancer and normal tissue from the NCI-MD case control study to ask this question in human lung cancer. To investigate the second part of this hypothesis, we are conducting a similar protocol but instead we are subjecting the samples to miRNA sequencing. Expression differences in isomiR formation will be further characterized for functional significant in follow up studies.