GeneLab Core

基因实验室核心设施为所有中心提供分子生物学支持、试剂和服务。 实验性传染病研究（CEIDR）。基因实验室服务包括一般 支持和最先进的下一代测序（NGS）和生物信息学支持服务。的 路易斯安那州立大学（LSU）和杜兰国立大学（Tulane National）的所有CEIDR教师都非常重要 灵长类动物研究中心（TNPRC）是相对较新的（一年）基因实验室最先进的服务， 致力于NGS和生物信息学。这些活动是在达成协商一致决定后开始的， CEIDR的研究人员认为，正在进行的NIH资助的研究迫切需要他们 项目，以及整个路易斯安那州的NIH资助的社区。NGS设备包括寿命 技术公司配备Ion PGM和Ion质子测序仪的设备套件，以及 配备数字PCR功能的Quant Studio Flex系统。GeneLab NGS活动的重点是 病毒和细菌基因组的确认和测序，以及病原体和 宿主-病原体相互作用期间宿主转录的变化。提供生物信息学支持服务 通过与计算和技术中心（CCT）和路易斯安那州的合作， 生物医学研究网络（LBRN），由NIH：NIGMS INBRE资助P20 GM 103424支持。 基因实验室目前拥有5名博士水平的科学家，一名研究助理，两名计算机科学 研究生和两名本科生工作者。此外，James Cavalcoli博士， 密歇根大学的生物信息学核心安阿伯担任顾问， 建议和支持，以及最先进的生物信息学知识资源。基因实验室核心 满足了路易斯安那州高度未满足的需求，因为没有其他类似的支持核心实验室 基因实验室在NGS和生物信息学方面的努力与NIH主任的路线图是一致的， 它强调生物学和传染病研究的计算方法。

The GeneLab Core Facility provides molecular biology support, reagents and services to all Centerfor Experimental Infectious Disease Research (CEIDR) investigators. GeneLab sen/ices include general support and state-of-the art next generation sequencing (NGS) and bioinformatics support services. Of primary importance to all CEIDR faculty at Louisiana State University (LSU) and the Tulane National Primate Research Center (TNPRC) is the relatively new (one year) GeneLab state-of-the-art services, which are devoted to NGS and bioinformatics. These activities were started after consensus decisions were reached among CEIDR researchers that they were critically needed by ongoing NIH-funded research projects, as well as the entire Louisiana-based NIH-funded community. NGS equipment includes a Life Technologies, Inc. equipment suite with an Ion PGM and Ion Proton Sequencing Machines, as well as a Quant Studio Flex system equipped with digital PCR capabilities. GeneLab NGS activities are focused on confirmation and sequencing of viral and bacterial genomes, as well as the determination of pathogen and host transcriptional changes during host-pathogen interactions. Bioinformatics support services are provided through a collaboration with the Center for Computation and Technology (CCT), and the Louisiana Biomedical Research Network (LBRN), which is supported by the NIH: NIGMS INBRE grant P20GM103424. GeneLab is currently staffed with 5 PhD-level scientists, one research associate, two computer science graduate students and two undergraduate student workers. In addition, James Cavalcoli, PhD, Director of the Bionformatics Core at the University of Michigan, Ann Arbor serves as a consultant providing expert advice and support, as well as a resource for state-of-the art bioinformatics knowledge. The GeneLab Core fulfills a highly unmet need in the State of Louisiana, since there is no other similar support Core laboratory in the state.GeneLab's efforts in NGS and bioinformatics are consistent with the NIH Director's roadmap, which emphasizes computational approaches to biology and in our infectious disease research.