Recovery of HIV Monoclonal Antibody Inventory Lost after Hurricane Sandy

描述（由申请人提供）：自从她在1989年发表了第一篇描述HIV-1的人类单克隆抗体（mab）的论文以来，Zolla-Pazner博士已经将这些试剂提供给HIV研究界。迄今为止，已经制备了100多种单抗，并进行了免疫化学和功能鉴定。其中包括从感染不同进化支（A、B、C、D、CRF01_AE、CRF02_AG等）的个体细胞中提取的人单克隆抗体，这些单克隆抗体对p24、gp41（集群1和2）和gp120 （V2、V3、C2、C5、CD4bs和V2V3四元中和表位）具有特异性。此外，已经产生了对CMV、MuLV gp70、炭疽保护抗原（PA）、霍乱毒素B和细小病毒b9特异性的单克隆抗体，这些单克隆抗体经常被用作抗hiv单克隆抗体的阴性对照。多年来，纽约大学艾滋病研究中心一直支持向艾滋病研究界提供这些单克隆抗体（~200毫克/年）。自2008年以来，提供这些试剂的部分资金已通过Fisher BioServices （FBS-0022C-05）的分包合同提供，该分包合同直接与NIH签订，以维持NIH艾滋病研究和试剂库计划（ARRRP）。超过100条单克隆抗体生产线被冷冻在我们的实验室里。从这些细胞系中产生上清液并储存，直到储存库或个别科学家提出要求。上清液作为纯化所需IgG单克隆抗体的原料，在运输前进行定量和质量控制测试。12年10月29日超级风暴桑迪造成电力中断时，我们实验室储存了来自38个单克隆抗体产生细胞系的62升上清。必须恢复这一清单，以便继续向艾滋病毒研究界提供这些试剂。此申请请求支持以补充该库存，这需要购买许多细胞系生长所需的大量供应品，以及纯化，定量

DESCRIPTION (provided by applicant): Since the publication of her first paper describing human monoclonal antibodies (mAbs) to HIV-1 in 1989, Dr. Zolla-Pazner has made these reagents available to the HIV research community. To date, more than 100 mAbs have been made and immunochemically and functionally characterized. These include human mAbs derived from the cells of individuals infected with diverse clades, (A, B, C, D, CRF01_AE, CRF02_AG, etc.) with specificities for epitopes in p24, gp41 (clusters 1 and 2), and gp120 (V2, V3, C2, C5, CD4bs, and the V2V3 quaternary neutralizing epitope). In addition, mAbs have been generated that are specific for CMV, MuLV gp70, protective antigen (PA) of anthrax, cholera toxin B, and parvovirus Bl 9 which are frequently used as negative controls for anti-HIV mAbs. For many years, the provision of these mAbs (~200 mg/year) to the AIDS research community was supported by the NYU Center for AIDS Research. Since 2008, partial funding for the provision of these reagents has been provided via a subcontract from Fisher BioServices (FBS-0022C-05) which contracts directly with the NIH to maintain the NIH AIDS Research and Reagent Repository Program (ARRRP). Over 100 mAb-producing lines are frozen in our lab. Supernatants are produced from these lines and stored until requested by the Repository or individual scientists. The supernatants serve as the source material for purifying the needed IgG mAbs which are quantified and subjected to quality control testing prior to being shipped. Sixty-two liters of supernatants from 38 mAb-producing cell lines were in storage at our lab when electricity was lost as a result of Super Storm Sandy (10/29/12). This inventory must be restored in order to continue the provision of these reagents to the HIV research community. This application requests support to replenish this inventory which requires the purchase of the extensive supplies needed for the growth of the many cell lines, and the purification, quantitation and quality assurance testing of the mAbs, along with the salary of a full time, highly qualified and experienced Senior Research Technician who has worked in the PI'S lab for >20 years.