Analytical Imaging Core (Core B)

项目摘要 分析成像核心（核心B）将继续服务于本计划项目的四个项目。 这个核心的长期目标是为各个项目的需要提供基于图像的支持 程序、蛋白质分析和化学生物学。 这一核心的具体目标是：1）提供一个全面的成像设施， 形态学研究中的研究人员需要分析自噬中年龄相关变化的作用， 不同的细胞和动物模型; 2）与个人项目的成员一起工作， 在他们的蛋白质分析方法的实验环境中实施;以及3）启动 开发基于调节自噬途径的靶向治疗方法，用于在不同的 细胞和动物模型。 核心的组成部分是：1）成像股：它已经并将继续提供以下方面的咨询意见： 样品制备/处理以及分析成像的技术和程序， 专门的成像程序，并协助解释形态学图像; 2） 创新单位：探索，测试和实施蛋白质修饰分析和蛋白质合成的程序。 使用结构和化学生物学开发自噬的化学调节剂。 该中心提供的服务包括：一系列全面的分析成像程序（直接和 间接免疫荧光、真实的时间荧光显微术、共聚焦荧光、常规和 冷冻电子显微镜和免疫金），获得成像仪器，和股票的共同 用于成像程序的试剂（抗体、探针和荧光染料）。提供的新服务 在此期间，将包括断层扫描，免疫金/银增强完整细胞标记，体内 成像、蛋白质聚集体分离和开发、CMA激活剂的表征和分布。 关键人员是主任、专职技师、专家级技术人员的成像核心 他是化学医学专家和靶向治疗专家。董事们将监督 核心的所有活动，提供建议并协助解释结果。技术人员将准备 并处理形态学研究所需的样品，并维持岩心所需的用品和试剂。 相关性：成像程序和蛋白质分析是必不可少的，共同的所有四个项目在这一领域。 程序，因为它们允许在整个细胞或器官中研究感兴趣的细胞过程。的 程序的集中化优化了成本效率，保证了程序的统一性，并允许整合 从不同群体获得的数据。将化学生物学成分加入核心 将促进基于自噬调节的抗衰老疗法的发展。

Project Summary The Analytical Imaging Core (Core B) will continue serving the four projects of this Program Project. The long-term goal of this core is to provide support for the needs of the individual projects on image-based procedures, protein analysis and chemical biology. The specific aims of this core are: 1) to provide a comprehensive imaging facility that will assist the investigators in the morphological studies required to analyze the role of age-related changes in autophagy in different cellular and animal models; 2) to work with the members of the individual projects toward the implementations in their experimental settings of methods for protein analysis; and 3) to initiate the development of targeted therapeutics based on modulation of autophagic pathways for testing in the different cellular and animal models used by the projects. The components of the core are: 1) The Imaging unit: That has and will continue providing advice on sample preparation/processing and techniques and procedures for analytical imaging, performing the specialized imaging procedures, and assisting with the interpretation of morphological images; 2) The innovation unit: That explores, tests and implements procedures for protein modification analysis and for the development of chemical modulators of autophagy using structural and chemical biology. The services offered by the core include: a comprehensive array of analytic imaging procedures (direct and indirect immunofluorescence, real time fluorescence microscopy, confocal fluorescence, conventional and cryoelectron microscopy and immunogold), access to the imaging instrumentation, and a stock of common reagents (antibodies, probes and fluorescent dies) for the imaging procedures. The new services available during this period will include tomography, Immunogold/silver enhancement for intact cells labelling, in vivo imaging, protein aggregates islation and development, characterization and distribution of CMA activators. The key personnel are the directors, a dedicated technician, the expert technical staff of the imaging core at Einstein, an expert in chemical medicine and an expert in targeted therapeutics. The directors will supervise all of the activities of the core, offer advice and assist with result interpretation. The technical staff will prepare and process samples for morphological studies and maintain the supplies and reagents required for the core. Relevance: Imaging procedures and protein analysis are essential and common to all four projects in this program as they allow the study of the cellular process of interest in the whole cells or organs. The centralization of the procedures optimizes cost efficiency, guarantees their uniformity and allows for integration of the data obtained by the different groups. The incorporation of a chemical biology component to the core will facilitate development of anti-aging therapeutics based on autophagy modulation.