SR Trans

转基因和基因敲除小鼠模型已成为索尔克研究计划的一个组成部分。转基因核心致力于提供尖端技术来创建这些模型。核心服务包括将DNA构建体显微注射到单细胞期胚胎中，将基因靶向小鼠胚胎干细胞显微注射到囊胚中，慢病毒感染和注射，体外受精（IVF），冷冻保存和小鼠品系的再衍生。此外，核心提供注射人类胚胎干（hES）细胞系和诱导多能干（IPS）细胞系到免疫缺陷小鼠形成畸胎瘤。 转基因核心开发新的技术和应用，提供直接访问个人处理转基因小鼠的知识，并提供研究合作的潜力。自1994年以来，已有100多篇出版物基于转基因核心创建的转基因小鼠品系。 所有CCSG支持的核心都被组织成一个组：基础研究。 此共享资源在摘要ID中被归类为类别（1.03）转基因动物设施

Transgenic and knockout mouse models have become an integral part ofthe research programs at Salk. The Transgenic Core is dedicated to providing access to cutting-edge technologies to create these models. Core services include microinjection of DNA constructs into one-cell stage embryos, microinjection of gene targeted mouse embryonic stem (ES) cells into blastocysts, lentiviral infection and injection, in-vitro fertilization (IVF), cryopreservation and rederivation of mouse lines. In addition, the Core offers injection of human embryonic stem (hES) cell lines and induced pluripotent stem (IPS) cell lines into immunodeficient mice to form teratomas. The Transgenic Core develops new techniques and applications, provides immediate access to individuals with knowledge of dealing with transgenic mice, and offers the potential for research collaborations. More than 100 publications were based on genetically altered mouse lines created by the Transgenic Core since 1994. All CCSG supported core are organized into one group: Basic Research. This shared resource is categorized as category (1.03) Transgenic Animal Facility in summary ID