LAB CORE

人乳头瘤病毒（HPV）感染在人宫颈癌中的病因学作用，以及人疱疹病毒-8 （HHV-8/KSHV）在卡波西肉瘤中的病因学作用是明确的。在HIV-1合并感染引起的免疫功能障碍下，这两种恶性肿瘤的发病率和严重程度都加剧了。在艾滋病毒-1发病率仍然很高的撒哈拉以南非洲地区，即使艾滋病毒-1不是致病因素，即使通过抗逆转录病毒治疗控制了抗逆转录病毒疾病，感染人乳头瘤病毒或KSHV/HHV-8也可能导致恶性肿瘤的可能性增加。在海洋路癌症研究所（ORCI），卡波西肉瘤和宫颈癌分别是男性和女性的主要癌症，因此了解该地区癌症发展的分子流行病学和病毒基因型，并确定针对这些病毒的潜在有效免疫反应，以开发潜在的疫苗或预防策略至关重要。ORCI与内布拉斯加州大学的两个分支机构之间建立了伙伴关系，以调查这些问题并提高ORCI的研究能力。这一伙伴关系将为培训和信息/技术交流提供环境，从而提高坦桑尼亚医疗从业人员的知识和研究能力。该核心将由内布拉斯加州病毒学中心和ORCI实验室科共同负责，为内布拉斯加州和坦桑尼亚受训人员联合开展的两个研究部门提供技术支持。该核心还将提供能力建设，促进培训和教育机会，通过讲习班和实践培训活动向两个地点的坦桑尼亚人转让技术和专门知识。具体目标是：1)提供精心策划的患者数据收集和存储，再加上患者样本采购，现场处理和长期档案存储，为联盟的各个方面；2)制定和实施国内HPV和HHV-8的诊断方法，重点分别是基于pcr的菌株分化和血清学方法；3)作为坦桑尼亚医学研究员和其他人员的教育/研究培训中心，以支持癌症研究合作并加强地方和区域专业知识。

The etiologic role of human papilloma virus (HPV) infection in human cervical carcinoma, and of human herpesvirus-8 (HHV-8/KSHV) in Kaposi's sarcoma, is clear. Both malignancies are exacerbated in incidence and severity under the immunological dysfunction induced by HIV-1 co-infection. In regions of sub-Saharan Africa where HIV-1 incidence remains high, infection with either HPV or KSHV/HHV-8 can lead to increased likelihood of malignancy even if HIV-1 is not a contributor and even if retroviral disease is controlled through antiretroviral treatment. At the Ocean Road Cancer Institute (ORCI), Kaposi's sarcoma and cervical cancer are the leading cancers among males and females, respectively, so it is critical to understand the molecular epidemiology and viral genotypes involved in cancer development in the region and to define any potentially effective immune responses against these viruses for potential vaccines or preventive strategies. A partnership between ORCI and two branches of the University of Nebraska has been established to investigate these issues and to increase ORCl's research capacity. The partnership will provide an environment for training and information/technology exchange that will increase the intellectual and research capacity among Tanzanian medical practitioners. This Core will be divided between the Nebraska Center for Virology (NCV) and the ORCI Laboratory Section to provide technical support for the two research arms to be conducted jointly by trainees in Nebraska and Tanzania. This Core will also provide capacity building and foster training and educational opportunities for technology and expertise transfer to Tanzanians in both locations through workshops and hands-on training exercises. Specific aims are to: 1) provide carefully curated patient data collection and storage, coupled with patient sample procurement, on-site processing, and long-term archival storage for all aspects of the Consortium; 2) develop and implement in-country diagnostics for HPV and HHV-8 focused on PCR-based strain differentiation and serological methods, respectively; and 3) serve as an education/research training center for Tanzanian medical fellows and others to support cancer research collaborations and enhance local and regional expertise.