Acquisition of a 700 MHz NMR Spectrometer

这一请求是为了获得一台4通道700兆赫核磁共振光谱仪的资金 用于大分子结构和组装的溶液研究。该仪器将配备一个 高灵敏度三重共振（1H，13C，15N）TCI冷冻探针，将大大提高灵敏度， 允许分析具有有限溶解度的样品。目前还没有专门用于 俄勒冈州州立大学的大分子NMR。大分子NMR组先前利用了 Bruker DRX 600 MHz仪器，1994年购买，但已无法使用并封存 自2012年2月以来。新仪器对于NIH支持的几个俄勒冈州州立大学的需求至关重要 来自俄勒冈州健康科学大学的大学研究人员和其他生物医学研究人员， 俄勒冈州，波特兰州立大学和该地区较小的机构。拟议的主要研究包括 天然无序蛋白质在大分子组装中的作用，特别是动力蛋白马达蛋白 复杂，蛋白脱酰胺在白内障透镜和老化，蛋白质动力学的作用，酶 对包括HIV和ALS在内的各种疾病的催化和机制研究。持续的生产力和 增长，以满足现有的核磁共振组和扩大用户群的需求依赖于此 采集新仪器将被安置在最近翻新的空间在校园范围内的核磁共振 该设施目前配备了专用于天然产品和小有机产品的仪器 分子。将其纳入现有的核磁共振中心将确保最佳的维护和使用 这个尖端仪器俄勒冈州州立大学致力于建立一个强大的校园范围内的核磁共振 核心，结合小分子和生物分子核磁共振设施，并支持这一建议， 为文书费用提供实质性匹配，并为业务费用提供经常性支持， 建立用户基础，并确保仪器广泛使用，即使是对学生的访问有限， 经费Barbar博士的蛋白质NMR专业知识和在获取多用户仪器方面的记录， 有兴趣促进一个广泛使用的设施，使重要的，生物医学相关的研究， 她非常适合领导这项工作这次购买将是第一次在校园里，将有一个 对俄勒冈州州立大学和参与机构的基础设施的直接影响，因为它将 实现尖端科学，促进高度合作的生物医学研究，并支持充满活力的 俄勒冈州的研究环境。

This request is for funds to acquire a 4-channel 700 MHz nuclear magnetic resonance (NMR) spectrometer for solution studies of macromolecular structures and assemblies. The instrument will be equipped with a high-sensitivity triple resonance (1H, 13C, 15N) TCI cryoprobe that will substantially enhance sensitivity and permit analysis of samples with limited solubility. There is currently no high field instrument dedicated for macromolecular NMR at Oregon State University. The macromolecular NMR group previously utilized a Bruker DRX 600 MHz instrument that was purchased in 1994 but has been non-functional and mothballed since February 2012. The new instrument is critical for the needs of several NIH-supported Oregon State University researchers and other biomedical researchers from Oregon Health Science University, University of Oregon, Portland State University and smaller institutions in the region. Proposed major studies include the role of natively disordered proteins in macromolecular assembly, particularly in the dynein motor protein complex, protein deamidation in cataractous lens and aging, the role of protein dynamics in enzyme catalysis and mechanistic studies on various diseases including HIV and ALS. Continued productivity and growth to meet the needs of the existing NMR group and the expanding user base depend on this acquisition. The new instrument will be housed in the recently renovated space in the campus-wide NMR facility that is presently equipped with instrumentation dedicated to natural products and small organic molecules. Its incorporation into a pre-existing NMR center will ensure optimal maintenance and usage of this cutting-edge instrument. Oregon State University is committed to building a strong campus-wide NMR core that unites small molecule and biomolecular NMR facilities, and is supporting this proposal with a substantial match towards the cost of the instrument and recurring support towards operating costs to help build the user base and ensure the instrument is widely accessible even to students with limited access to funding. Dr. Barbar's protein NMR expertise and track record in acquiring multi-user instruments and interest in promoting a widely accessible facility enabling significant, biomedically relevant research make her uniquely suited to lead this effort. This purchase will be the first of its kind on campus and will have an immediate impact on the infrastructure at Oregon State University and participating institutions as it will enable cutting-edge science, foster highly collaborative biomedical research, and support the vibrant research environment in the State of Oregon.