Molecular Pathology Core

核心摘要/摘要 分子病理学核心为超级基金研究计划的研究人员提供 评估分子和形态变化所需的设备和技术专长 暴露于复杂环境污染物后的细胞、组织和器官。核心房屋州- 最先进的设备，包括一台自动组织处理器，一台石蜡包埋中心，两台自动 显微镜、低温恒温器、软组织切片振动器、带投影的多头光学显微镜 能力，激光捕获显微解剖系统，以及带有分析软件的载玻片扫描仪 形态结构的鉴定和量化。分子病理学核心由一名 主任、组织学家和组织技术员，他们在组织病理学和免疫细胞化学方面有专长 方法包括固定、脱水、包埋、切片、组织学染色、免疫标记、高密度扫描。 分辨率成像和定量图像分析。设施工作人员提供样例中的全方位服务 准备，在成像和图像分析方面提供帮助，并为正在进行或未来的工作提供咨询 研究项目。此外，该中心还为学生和 使用中央可用设备的调查人员。 分子病理学核心的广泛、长期的目标是对检测做出贡献， 诊断和预防因接触复杂环境污染物而引起的疾病。这么长的时间- 任期目标将通过以下具体目标实现：具体目标1.提供高质量、低成本的 为超级基金研究计划中的研究项目提供有效的分子病理学服务。 具体目标2.进一步整合分子病理学核心和LEDUC生物成像的方法学 设施，里面有电子显微镜、荧光显微镜和共焦显微镜。具体目标3.确定 特定协议中的瓶颈，并设计创新的解决方案，以改进提议的管道 从实验到结果的公布。具体目标4.提供组织加工、切片、 组织学染色、免疫标记、高分辨率成像和定量图像分析。预期中的 这项工作的成果包括识别分子标记和特定的病理变化 暴露于复杂环境污染物后的细胞和组织形态。结果和 已开发的方法将是超级基金研究中个别研究项目的关键 规划并促进环境污染物和混合物的循证风险评估。在……里面 此外，接受分子病理学核心培训的学生将配备跨学科工具 以及解决环境科学未来问题的方法。

CORE SUMMARY/ABSTRACT The Molecular Pathology Core provides researchers in the Superfund Research Program with equipment and technical expertise necessary for the evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. The Core houses state- of-the-art equipment, including an automated tissue processor, a paraffin embedding center, two automated microtomes, a cryostat, a vibratome for soft-tissue sectioning, a multiheaded light microscope with projection capabilities, a system for laser capture microdissection, and a slide scanner with analysis software for the identification and quantification of morphological structures. The Molecular Pathology Core is staffed by a director, a histologist and a histotechnician, who have expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high- resolution imaging, and quantitative image analysis. Facility staff provides all-inclusive services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects. In addition, the core established an extensive training program for students and investigators who use the centrally available equipment. The broad, long-term objective of the Molecular Pathology Core is to contribute to the detection, diagnosis, and prevention of disease caused by exposures to complex environmental contaminants. This long- term goal will be achieved through the following specific aims: Specific Aim 1. Provide high-quality, cost- effective services in molecular pathology for the research projects in the Superfund Research Program. Specific Aim 2. Further integrate methodologies in the Molecular Pathology Core and the Leduc Bioimaging Facility, which houses electron, fluorescence, and confocal microscopes. Specific Aim 3. Identify the bottlenecks in specific protocols and design innovative solutions to improve the pipeline from the proposed experiments to publication of the results. Specific Aim 4. Provide training in tissue processing, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. The expected outcome from this work includes the identification of molecular markers and specific pathological changes in cell and tissue morphology following exposure to complex environmental contaminants. The results and the developed methodologies will be essential to the individual research projects in the Superfund Research Program and contribute to evidence-based risk assessment of environmental contaminants and mixtures. In addition, students who are trained in the Molecular Pathology Core will be equipped with interdisciplinary tools and approaches to tackle future problems in the environmental sciences.