MR predictors of infection, inflammation, and structural lung damage in CF

描述（由申请人提供）：目前对患有囊性纤维化（CF）的婴幼儿肺部疾病的检测和监测是有限的，并且是该人群护理和研究进步的障碍。在这个建议中，我们假设，磁共振成像（MRI）的肺和肺循环有可能作为一个敏感的和便携式的工具来监测早期CF肺疾病，肺灌注和/或结构的变化可以与外周生物标志物代谢组学和蛋白质组学方法确定。该提案将MRI技术的进步与代谢组学和蛋白质组学的新兴技术结合起来，将新的MR成像与候选和新的途径分析联系起来。我们将检查肺血流的三个方面（总和局部肺灌注、血管阻力和肺动脉侧支血流），并与MRI和CT肺结构评估进行比较。这些研究将在6个月至3岁的CF和非CF对照中进行，并在CF患者中重复测量。将CF与非CF条件（包括氧化状态、嘌呤能信号传导和葡萄糖代谢）分离的候选代谢组学途径将是初始生物标志物分析的焦点，先进的生物信息学技术应用于定义代谢组学和成像之间的新关系。(End摘要）

DESCRIPTION (provided by applicant): Detection and monitoring of lung disease in infants and toddlers with cystic fibrosis (CF) is currently limited, and is a barrier to advancements in care and research for this population. In this proposal, we hypothesize that magnetic resonance imaging (MRI) of the lung and pulmonary circulation has the potential to serve as a sensitive and portable tool to monitor early CF lung disease, and that changes in pulmonary perfusion and/or structure can be correlated with peripheral biomarkers identified by metabolomics and proteomic methodology. The proposal couples advancements in MRI techniques with the emerging technologies of metabolomics and proteomics, linking new MR imaging to candidate and novel pathway analysis. We will examine three aspects of pulmonary blood flow (total and regional pulmonary perfusion, vascular resistance, and aortopulmonary collateral blood flow) compared with structural assessment of the lung by MRI and CT. These studies will be performed in CF and non-CF controls age 6 months - 3 years, with repeated measures in CF patients. Candidate metabolomic pathways that segregate CF from non-CF conditions (including oxidative status, purinergic signaling, and glucose metabolism) will be the focus of initial biomarker analysis, with advanced bioinformatic techniques applied to define novel relationships between the metabolome and imaging. (End of Abstract)