Gene Transfer Core, NIDCR-DIR

原核注射服务：注射92个转基因构建体以产生221个转基因株系，平均周转时间为7周。 基于CRISPR的基因编辑敲除服务：GTC发起了基于CRISPR的基因编辑构建体的注射。 CRISPR构建体产生KO和KI小鼠的平均效率分别为43%和3%。这项服务共产生了27条线路。 冷冻保存和IVF服务：来自67只小鼠模型的精子被冷冻保存，供17名研究者使用。23个精子管被解冻，用于63个IVF疗程。根据研究人员向精子冷冻保存服务部门提出的要求，成功重新建立了23个小鼠模型。 ES细胞注射服务：注射5个项目的6个克隆，产生27只嵌合小鼠，平均周转时间为4周。该服务的终点是将这些嵌合体提供给PI。使用这些嵌合体，通过PI产生2个杂合小鼠模型。 基因分型服务：3名研究者对1215份尾部活检标本进行纯化并用于PCR分析进行基因分型。 正如其他ZIA报告所示，2015财年发表了许多基于GTC生成的小鼠模型的期刊文章。

Pronuclear injection service: 92 transgenic constructs were injected to generate 221 transgenic lines with an average turnaround time of 7 weeks. CRISPR-based gene editing knockout service: GTC initiated injections for CRISPR-based gene editing constructs. The average efficiency for generating KO and KI mice for CRISPR constructs was 43% and 3%, respectively. A total of 27 lines were generated from this service. Cryopreservation and IVF services: Sperms from 67 mouse models were cryopreserved for 17 investigators. 23 sperm straws were thawed to use for 63 IVF sessions. 23 mouse models were successfully re-established per investigators request from the sperm cryopreservation service. ES cell injection service: 6 clones from 5 projects were injected to generate 27 chimeric mice with an average turnaround time of 4 weeks. The endpoint of this service was to provide these chimeras to PI. Using these chimeras, 2 heterozygous mouse models were generated by PIs. Genotyping service: 1215 tail biopsies were purified and used for genotyping by PCR analysis for 3 investigators. As indicated in others ZIA reports, numerous journal articles were published in FY15 based on the mouse models generated by the GTC.