Leica TCS SP8 Confocal Microscope System

 描述(由申请人提供)：本申请的目标是获得最先进的激光扫描共聚焦显微镜，以支持杜兰国家灵长类动物研究中心(TNPRC)由NIH资助的11名研究人员的研究。TNPRC是NIH支持的国家资源，其使命是整合生物医学研究的研究、培训和服务，通过基础和应用生物医学研究来改善人和动物的健康。现有的多用户共焦系统是2002年购买的徕卡TCS SP2，它已经连续运行了十多年，为研究界提供了出色的服务。虽然徕卡TCS SP2在购买时是最先进的，但该系统不再满足我们在多标签成像研究、光敏性、成像质量、速度和多功能性方面的需求。此外，徕卡将在明年停止为SP2提供服务。在这份申请中，我们介绍了11名研究人员(8名主要用户和3名次要用户)的研究项目，他们的工作在TNPRC进行。这些项目主要侧重于传染病和免疫学，这是TNPRC的特殊专长领域，吸引了国家和国际合作伙伴。每个用户都有NIH资助的丰富历史和对先进成像技术的需求。我们建议购买一台带有倒置显微镜的徕卡TCS SP 8五通道共焦显微镜成像系统，带有AOBS的6条强激光线路(二极管：405 nm，蓝色Ar：458,488,514 nm，黄色二极管：561 nm，红色Heene：633 nm)，以及两个AOTF(405 nm和可见光)，3个混合GaAsP光谱探测器和2个HS PMT，并且易于使用，所有这些都是用户项目必不可少的高级软件。与市场上其他首屈一指的系统相比，我们认为徕卡TCS SP 8在质量、选项、服务和价格方面提供了最好的整体系统。它还具有很高的灵活性，能够根据用户的需求提供新的服务。这种新的共焦显微镜系统不仅对申请中列出的主要和次要用户至关重要，而且对于TNPRC作为使用非人类灵长类进行生物医学研究的国家资源的使命也是必不可少的。更新、更可靠、更复杂、更多功能的仪器的出现，可能会吸引更多的研究人员，这些研究人员需要这种技术，从而导致新的合作，进一步扩大TNPRC的非人类灵长类研究的范围。

 DESCRIPTION (provided by applicant): This goal of this application is to acquire a state-of-the-art laser-scanning confocal microscope to support the research of 11 NIH-funded investigators at the Tulane National Primate Research Center (TNPRC). The TNPRC is an NIH-supported national resource with a mission that integrates research, training and service in biomedical research to improve human and animal health through basic and applied biomedical research. The existing multiuser confocal system, a Leica TCS SP2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. While the Leica TCS SP2 was state-of-the-art when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity, imaging quality, speed, and versatility. Furthermore, Leica will cease to provide service for the SP2 within the next year. In this application we present research projects from 11 investigators (8 Major users and 3 Minor users) whose work is performed at the TNPRC. These projects are primarily focused on infectious diseases and immunology which are areas of exceptional expertise at the TNPRC and that attract national and international collaborators. Each of the users has a strong history of NIH funding and needs for advanced imaging technology. We propose purchasing a Leica TCS SP 8 five-channel confocal microscopy imaging system with an inverted microscope, 6 strong laser lines (diode: 405 nm, Blue Ar: 458, 488, 514 nm, yellow diode: 561 nm, and red HeNe: 633 nm) with AOBS, and two AOTF (405 nm and visible), 3 hybrid GaAsP spectral detectors and 2 HS PMT and easy to use advanced software all of which are essential to the projects of the users. Compared to other premiere systems on the market we feel the Leica TCS SP 8 offers the best overall system in terms of quality, options, service, and price. It also is highly flexible and will be able to provide new services as required by the users. This new confocal microscope system is essential not only to the major and minor users listed in the application but to the mission of the TNPRC as a national resource for biomedical research using nonhuman primates. The availability of a newer, more reliable, sophisticated and versatile instrument such as the SP 8 is likely to attract additional investigators that require ths technology leading to new collaborations further expanding the scope of nonhuman primate research at the TNPRC.