Targeted Analysis Resource

摘要(目标分析资源) 密歇根儿童健康暴露分析资源(M-CHEAR)实验室的主要目标 中心是为促进我们对环境影响的认识的大规模国家努力做出贡献 通过提供高质量的高级实验室支持，为各种有针对性的和 进行流行病学和临床研究的外部研究人员的生物样本中的非靶向分析 关于儿童健康的研究。M-CHEAR目标分析资源的总体目标是提供 外部调查人员对一系列具有高度敏感性、选择性和最新水平的 可有效应用于儿童健康流行病学和临床研究的生物标志物。这将是 通过利用密歇根大学的核心设施和其现代化的设施和设备 合作实验室(NSF国际)，提供我们已经拥有的高度敏感的测量方法 在这些环境中开发并成功地应用于正在进行的高影响的儿童流行病学研究 健康，执行并遵守标准操作程序和严格的质量保证/质量 控制流程，不断扩展我们的分析能力，以及有效的内部和外部 有效响应调查员和CHEAR计划需求的沟通。具体分析有待改进 提供的服务包括：1)对暴露于一系列环境中的生物标志物进行高通量痕量水平分析 暴露，包括邻苯二甲酸盐、双酚A(BPA)和其他酚(如三氯生、二苯甲酮-3、 二氯苯酚)、对羟基苯甲酸酯、多环芳烃(PAH)、阻燃化学品、持久性 和非持久性农药、多氯联苯、全氟烷基化学品、烟草 烟雾、金属/类金属和其他；2)与营养和代谢有关的标记物的靶向分析 因素包括矿物质营养素、维生素、植物雌激素、脂质、氨基酸、脂肪酸、胆汁酸、 氧化甾醇、植物甾醇等；以及3)额外的内源性目标检测的有效分析。 测量激素水平、免疫指标和炎症、氧化应激的标志物，以及 心理社会压力。我们将与开发核心密切合作，制定 有针对性地分析新的外源或内源标记以及测量这些标记的方法 新型生物基质中的标记。有针对性的分析资源也将及时高质量地进行 数据处理以识别潜在错误并最大限度地提高易用性，与其他CHEAR进行沟通 实验室中心可以利用彼此的能力和专业知识，并参与循环 评估以确定现有或新方法的重复性，并比较准确度和精密度 整个CHEAR实验室中心的具体方法。最后，我们将能够提供我们多样化和独一无二的 擅长分析、解释和应用各种生物标记物进行流行性感冒的流行病学研究 儿童健康调查人员和CHEAR计划。

ABSTRACT (Targeted Analysis Resource) The primary objective of the Michigan Children’s Health Exposure Analysis Resource (M-CHEAR) Laboratory Hub is to contribute to large-scale national efforts to advance our knowledge of the impact of the environment on children’s health by offering high-quality, advanced laboratory support for a wide assortment of targeted and untargeted analyses in biological samples from external researchers conducting epidemiology and clinical studies of children’s health. The overarching goal of the M-CHEAR Targeted Analysis Resource is to provide external investigators with highly sensitive, selective, state-of-the-art targeted analysis of a broad array of biomarkers that can be effectively applied to epidemiology and clinical studies of children’s health. This will be done through leveraging core facilities at University of Michigan and the modern facilities and equipment at its partnering laboratory (NSF International), offering highly sensitive measurement methods that we have already developed in these settings and successfully applied to ongoing high-impact epidemiologic studies of children’s health, implementing and adhering to standard operating procedures and strict quality assurance/quality control processes, continually expanding our analysis capabilities, and effective internal and external communications to respond efficiently to investigator and CHEAR program needs. Specific analyses to be offered include: 1) High-throughput trace level analysis of biomarkers of exposure to a range of environmental exposures, including phthalates, bisphenol A (BPA) and other phenols (e.g., triclosan, benzophenone-3, dichlorophenols), parabens, polycyclic aromatic hydrocarbons (PAH), flame retardant chemicals, persistent and non-persistent pesticides, polychlorinated biphenyls (PCBs), perfluoroalkyl chemicals (PFCs), tobacco smoke, metals/metalloids, and others; 2) Targeted analysis of markers related to nutrition and metabolic factors including mineral nutrients, vitamins, phytoestrogens, lipids, amino acids, fatty acids, bile acids, oxysterols, phytosterols, and more; and 3) Efficient analysis of additional targeted assays of endogenous measures such as hormone levels, immune indicators, and markers of inflammation, oxidative stress, and psychosocial stress. We will work closely with the Developmental Core in the development of methods for targeted analysis of new exogenous or endogenous markers, as well as methods for measurement of these markers in novel biological matrices. The Targeted Analysis Resource will also conduct timely and high-quality data processing to identify potential errors and maximize ease of use, communicate with other CHEAR Laboratory Hubs to capitalize on one another’s capabilities and expertise, and participate in round-robin assessments to determine reproducibility of existing or new methods and to compare accuracy and precision of specific methods across CHEAR Laboratory Hubs. Finally, we will be able to offer our diverse and unique expertise in analysis, interpretation and application of the various biomarkers to epidemiology studies of children’s health to investigators and the CHEAR program.