Spatiotemporal Control of Dynamic Notch Signaling with Subcellular Resolution

描述(申请人提供)：Noch信号是一种高度保守的细胞间通讯机制，在决定单个细胞在发育过程中的行为和命运方面发挥着核心作用。尽管对这些信号事件的了解迅速增加，但对细胞内受体信号的时空动态如何影响信号交换却知之甚少。为了应对这一挑战，我们提出了一种先进的纳米系统来模拟，能够以亚细胞分辨率在任何所需的位置和时间对特定细胞中的Notch信号进行同步实时监测和原位调节。利用这种新的纳米技术，我们首先确定了Notch受体的力诱导结构特征。我们还研究了神经干细胞中的Notch信号，以确定Notch信号在细胞群体中的时空分布如何影响单个细胞在发育过程中的最终命运。通过Notch的二进制信元通信。

DESCRIPTION (provided by applicant): Notch signaling is a highly conserved cell-to-cell communication mechanism, which plays a central role in defining individual cells' behaviors and fates during development. Despite rapidly increasing knowledge of these signaling events, little is known about how spatiotemporal dynamics of receptor signaling across the cell influence signal exchange. To address this challenge, we propose an advanced nanosystem that mimics, enabling simultaneous real-time monitoring and in situ regulation of Notch signaling in a particular cell at any desired location and time with subcellular resolution. Using this new nanotechnology, we first determine force-induced structural features of Notch receptors. We also explore Notch signaling in neural stem cells to determine how the spatiotemporal distribution of Notch signaling across a cell population influences the final fate of individual cels during development. Binary cell communication via Notch.