Elucidating the Mechanisms of Reverse Transcription and Recombination

频繁的重组极大地促进了艾滋病毒-1种群的多样性。我们已经研究了HIV-1重组的多个方面。我们利用在俄罗斯流行的毒株研究了A亚型HIV-1的重组潜力，我们发现重组发生得很频繁，可以很容易地对突变进行分类，产生对多种抗病毒药物具有耐药性的变异体。我们还研究了宿主限制因子APOBEC3G和APOBEC3F如何影响病毒复制，以及这些限制因子诱导的超突变是否在病毒进化中发挥作用。此外，我们还协助构建了可用于监测细胞间感染的复制依赖载体。这些研究将揭示对HIV-1复制机制的洞察。[对应于2011年10月艾滋病毒耐药计划现场访问报告中的HU项目1,2015年更名为艾滋病毒动态和复制计划]

Frequent recombination contributes significantly to the diversity of the HIV-1 population. We have studied multiple aspects of HIV-1 recombination. We have studied the recombination potential of subtype A HIV-1 using strains that are circulating in Russia, and we found that recombination occurs frequently and can easily assort mutation to generate variants resistant to multiple antivirals. We have also studied how host restriction factors APOBEC3G and APOBEC3F affect viral replication, and whether hypermutations induced by these restriction factors play a role in viral evolution. Additionally, we have assisted in the construction of replication-dependent vectors that can be used to monitor cell-to-cell infection. These studies will reveal insights into the replication mechanisms of HIV-1. [Corresponds to Hu Project 1 in the October 2011 site visit report of the HIV Drug Resistance Program, renamed the HIV Dynamics and Replication Program in 2015]