X/Q Band Pulsed ENDOR Spectrometer

 描述(由申请人提供)：这项提案的目的是在康奈尔大学建立现代脉冲电子-核双共振(Endor)能力，该能力将位于国家高级ESR技术生物医学中心(ACERT)。它将被康奈尔大学、罗切斯特大学和纽约州立大学布法罗分校的研究小组使用。ACERT拥有维护该设施所需的所有专业知识和技术能力。NIH资助的主要用户的研究项目包括：(1)含[4Fe-4S]-簇的自由基酶在二甲胺生物合成中的新化学的Endor研究；(2)Endor和电子自旋回声包络调制(ESEEM)研究，以探讨水合作用在膜融合中的作用；(3)Endor光谱学研究通过氧化还原和光化学介导信号转导的蛋白质；(4)抗癌药物抑制核糖核酸酶还原酶的机制；以及(5)多孔体的结构和动力学--新型光敏剂在癌症治疗和药物输送中的作用。来自少数用户的其他项目包括：(6)人工氢酶的结构和功能；(7)酶和电子转移蛋白的硝化；以及(8)脂质表面的水合作用。ACERT自2001年成立以来，为代表多个生物医学领域的全国多个研究小组提供EPR专业知识和服务。虽然目前在ACERT进行的EPR对于获得关于生物系统中的顺磁性辅助因子的结构和功能信息至关重要，如铁-硫团簇、铜活性中心等，但使用Endor进行的额外研究往往是必要的，但康奈尔大学没有这些用户。将康奈尔大学的Endor共享能力放在Acert将极大地提高参与这项提议的团体以及与Acert互动的其他团体的研究质量。他们将控制自己的恩多尔测量，这将成为他们日常研究的一部分。此外，重要的是，Endor和EPR研究将在相同的样本和相同的条件下同时进行。如主要/次要用户项目所述，Endor和EPR(例如ESEEM)在揭示所研究的生物系统中存在的未配对电子和核自旋之间相互作用的不同方面方面是相辅相成的。

 DESCRIPTION (provided by applicant): The aim of this proposal is to establish a modern pulsed Electron-Nuclear Double Resonance (ENDOR) capability at Cornell University to be located in the National Biomedical Center for Advanced ESR Technology (ACERT). It will be used by research groups at Cornell, University of Rochester and SUNY Buffalo. ACERT possesses all the necessary expertise and technical capability to maintain the facility. The NIH-supported research projects of the major users include: (1) ENDOR study of novel chemistry of a [4Fe-4S]-cluster- containing radical enzyme in diphthamide biosynthesis; (2) ENDOR and Electron-Spin Echo Envelope Modulation (ESEEM) to probe the role of hydration in membrane fusion; (3) ENDOR spectroscopy studies of proteins that mediate signal transduction through redox and photochemistry; (4) mechanism of inhibition of ribonuclease reductase by anti-cancer drugs; and (5) structure and dynamics of porphysomes - new photo- sensitive agents in cancer therapy and drug delivery. Additional projects from minor users include: (6) structure and function of artificial hydrogenases; (7) nitrification of enzymes and electron transfer proteins; and (8) hydration at lipid surfaces. ACERT, since its establishment in 2001 provides EPR expertise and service to many research groups nationally which represent many biomedical fields. While the EPR currently performed in ACERT has been crucial in obtaining structural and functional information on paramagnetic cofactors in biological systems, such as iron- sulfur clusters, copper active sites etc., additional studies using ENDOR were often necessary but not available at Cornell for these users. Having an ENDOR shared capability at Cornell to be housed in ACERT will dramatically improve the quality of research for the groups participating in this proposal, as well as others who interact with ACERT. They will have control of their ENDOR measurements, which would become part of their daily research. Also, importantly, ENDOR and EPR studies will be carried out at the same time on the same samples and under the same conditions. As described in projects of major/minor users, ENDOR and EPR (e.g. ESEEM) are complimentary to each other in terms of revealing different aspects of interactions between the unpaired electron and nuclear spins present in the biological system studied.