COBRE: Smooth Muscle Plasticity

在第三阶段的科布雷，我们将继续发展的主题焦点：平滑肌可塑性，通过 继续和加强在Cobre第一阶段和第二阶段发展的核心实验室服务 是包含多种细胞类型的复杂组织。将这些肌肉视为同种组织 是评估基因表达和基因表达中发生的变化的常用方法 如发育和衰老、糖尿病、重塑、对炎症的反应等 内华达大学的平滑肌生物学小组首创了对细胞进行分类的技术 使用细胞特异性报告器和表面抗原对平滑肌组织进行成分分析 全基因组对细胞特定转录本的评估。在特定细胞类型中非常突出的基因是 使用Fluidigm BioMark HD技术进行评估以确定细胞的数量变化 当平滑肌组织在发育或疾病状态下发生变化时，基因表达会发生变化。转录分析还伴随着对哪些转录本使用核糖体进行主动翻译的评估 侧写技术。我们还在创造特定基因上调或下调的细胞系。 为COBRE的主题重点提供的服务将由核心B提供(FACS/流式细胞术共享 资源实验室)和核心C(单细胞分子表达)。提供了详细的计划，以加强 在整个大学建立这些核心的用户基础，并为以下方面创建持久可持续的核心设施 该州的研究界。第三阶段将由一项试点赠款计划补充，该计划是 旨在通过大学内的其他几个项目增加核心的使用率。试点补助金将是 授予合格的调查人员，以使用核心技术获取试点数据，以便利用 核心将增强赠款提案的力度，并在为新的赠款提供资金时增加核心使用率。 第三阶段由一个行政核心(核心A)领导，该核心通过计划总监提供领导 和计划协调员，通过内部指导委员会进行质量控制，并通过 一名评估协调员和一个外部咨询委员会。

In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases I&II. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Program Director and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.