Precision Cell Isolation and Analysis Core

项目摘要-核心B 无论是体内的小鼠模型还是体外的人类模型，CMV感染都表明蛋白质在 炎性信号通路在潜伏期和再激活过程中调节致病表观遗传事件。 对巨细胞病毒和巨细胞病毒的体外模型进行稳健的表征将是确定机制的关键 途径，反过来可以告知如何再次激活在临床上发生的移植设置。这些项目 在这篇PPG中提出的一系列体外模型将提供有用的见解，进而 提高我们对这一过程的理解。要做到这一点，高度技术性和尖端的研究工具 需要各种不同的专门学科，这些学科不可能由任何一个调查员或 实验室。为此，我们组建了一支科学家团队，他们将提供必要的支持， 帮助项目负责人实现其既定的科学和机械目标的专业知识和指导， 用功能基因组学和蛋白质组学进行科学细胞分选。精密电池的长期目标 隔离和分析核心(核心B)是为项目负责人提供一站式服务，可以执行和 协调开展拟议研究所需的资源和技能，提供概念性的 体内和体外CMV潜伏期和再激活模型之间的机制统一 在方案项目中嵌入各种研究，同时保障和确保宝贵的生存能力 样本。这些服务将包括为荧光激活提供技术支持和专业知识 细胞分类、精密蛋白质组学和促进与校外合作者的互动，专注于 核酸分析和单细胞多参数表型。核心B将利用现有的机构 资产，但将激发一组特定的专门服务，重点放在 MCMV潜伏期和重新激活是其基础。这种方法在两个层面上都是创新的。首先，每一位 计划由不同小组执行的用于调查CMV潜伏期和 重新激活是新奇的。第二，将这一组核心能力聚集在一起，共同努力实现 共同的主题在这一领域也是新奇的。拟议的核心人员、监督和质量控制，以及 固有的效率和协同效应将确保按照最佳方式处理和转移样品 将确保项目负责人提议的工作受益于专门知识的汇编 否则可能无法对这种类型的调查做出贡献。

PROJECT SUMMARY - Core B Both in vivo murine models and ex vivo human models of CMV infection point to a role for proteins within the inflammatory signaling pathways in mediating causative epigenetic events during latency and reactivation. Robust characterization of in vitro models for both HCMV and MCMV will be essential in defining mechanistic pathways that can in turn inform how reactivation occurs clinically in the setting of transplantation. The projects proposed in this PPG encompass a spectrum of in vitro models that will provide useful insights that will in turn improve our understanding of this process. To do this, highly technical and sophisticated research tools are needed that require various specialized disciplines that cannot possibly be mastered by any one investigator or laboratory. For this reason, we have assembled a team of scientist who will provide the necessary support, expertise and guidance to help project leaders achieve their stated scientific and mechanistic goals, state-of- the science cell sorting with functional genomics and proteomics. The long-term goal of the Precision Cell Isolation and Analysis Core (Core B) is to provide Project Leaders with a `one-stop shop' that can perform and coordinate resources and skill sets necessary to carry out the proposed studies, providing a conceptual mechanistic unity between the in vivo and in vitro CMV models of latency and reactivation proposed in the various studies embedded in the program project, while safeguarding and ensuring the viability of the precious samples. These services will include providing the technical support and expertise for Fluorescence Activated Cell Sorting, Precision Proteomics, and facilitating interactions with off-campus collaborators focused on nucleic acid analysis and single-cell multi-parameter phenotyping. Core B will leverage existing institutional assets, but will galvanize a specific set of specialized services with a laser focus on the mechanisms that underlie MCMV latency and reactivation. This approach is innovative on two levels. First, each of the techniques planned to be performed by the various groups for the investigation of CMV latency and reactivation is novel. Second, bringing together this set of core competencies working collaboratively towards a common theme is also novel in this field. The proposed Core personnel, oversight and quality control, and the inherent efficiencies and synergies will ensure that samples are processed and transferred according to best practices, will ensure that the work proposed by the project leaders benefits from a compilation of expertise that might otherwise not be able to contribute to this type of investigation.