Histology Core Laboratory

病理学实验室（LP）的组织学（组织学）部分是致力于支持LP临床活动的核心设施。该实验室处理来自NIH和非NIH患者的各种组织标本，包括各种恶性肿瘤、艾滋病相关诊断标本、感染和监测活检。这包括登记在手术或诊所中切除的人体组织，处理组织，准备和染色载玻片，供LP的病理学家、临床研究员和住院医生进行显微镜检查。病理学住院医师接受LP病理学家助理（PA）的大体解剖技术培训，并学习评估大小标本，为组织学旋转期间的显微镜评估做准备。组织学实验室为外科病理学、血液病理学、死后病理学和细胞病理学部门以及专业/分子诊断部门提供服务。到目前为止，在截至7月的本财政年度期间，制备了约26，000个组织块，并从内部活检和手术病例中切割了超过172，000个载玻片。在此期间，LP的病理学家助理进行并培训住院医生进行大体分析，并为从2，600多个内部活检和手术组织中收集的组织选择最具诊断性的切片。在组织学中进行了超过53，000次特殊染色，在免疫组织化学中进行了大约24，000次染色，这些染色来自内部和提交的病例。按照目前的速度，组织学预计将削减约230,000幻灯片和执行115,500特殊和IHC染色的整个财政年度。该实验室还处理NIH或非NIH患者从外部实验室提交的切片和组织块。到目前为止，该实验室处理了来自2,100多个病例的93，000多张咨询切片和2，500块，这些病例提交给LP的病理学家，供NIH临床中心收治的患者进行临床咨询或审查或进入方案。虽然超过95%的工作是致力于临床职责，组织学实验室也进行组织块的再切割，并通过安排在NCI的研究项目做一些常规染色。该活动与临床运营部的组织研究请求职能协调。在本财政年度，从1,600多个ffpe组织块中重新切割的8,500多个未染色和染色的载玻片和150多个组织剃刮管被制备并分发给NIH范围内的研究者。组织学实验室不进行主要研究，但支持许多研究者的活动。

The Histopathology (Histology) section of the Laboratory of Pathology (LP) is a core facility devoted to supporting the clinical activities of LP. The laboratory processes a large variety of tissue specimens from NIH and non-NIH patients, including a wide variety of malignancies, AIDS-related diagnostic specimen, infections, and surveillance biopsies. This consists of accessioning human tissues removed at surgery or in clinics, processing the tissues, preparing and staining slides for microscopy by LP's pathologists, clinical fellows and residents. Pathology residents are trained by LP's Pathologists Assistants (PAs) in techniques of gross dissection and learn to evaluate large and small specimens in preparation for microscopic evaluation during Histology rotations. The Histology lab serves the Surgical Pathology, Hematopathology, Postmortem Pathology and Cytopathology sections as well as the Specialized / Molecular Diagnostics Unit. To date during this fiscal year through July, approximately 26,000 tissue blocks were prepared and over 172,000 slides were cut from inhouse biopsy and surgical cases. During this period, LP's Pathologists Assistants performed and trained residents in gross analysis and selection of the most diagnostic sections for tissues collected from over 2,600 inhouse biopsy and surgical tissues. Over 53,000 special stains were performed in Histology and approximately 24,000 stains in Immunohistochemistry from inhouse and submitted cases. At the current rate, Histology is projected to cut about 230,000 slides and perform 115,500 special and IHC stains for the full fiscal year. The lab also processes slides and blocks submitted from outside laboratories for NIH or non-NIH patients. So far this fiscal year, the laboratory processed processed over 93,000 consult slides and 2,500 blocks from over 2,100 cases that were submitted to LP's pathologists for clinical consultation or review for patients being admitted to the NIH Clinical Center or entry on protocol. Although more than 95% of effort is devoted to clinical duties, the Histology Laboratory also performs recuts of tissue blocks and does some routine staining for research projects in NCI by arrangement. This activity is coordinated with the tissue research request function of Clinical Operations. For this fiscal year, more than 8,500 unstained and stained slides and more than 150 tissue shaving tubes, recut from more than 1,600 ffpe tissue blocks, were prepared and issued to investigators NIH-wide. The Histology Lab does not conduct primary research but supports the activities of many investigators.