SBIR Topic 359 Phase I: Highly-Efficient Isolation and Fidelity Differentiation of Exosomes (HIFiDE) from Bodily Fluids Kit

外切体封装和运输由它们的起源细胞产生的各种各样的分子，这一过程现在被认为是一种细胞信号形式。外切体信号在细胞类型和物种中很常见，但它在含有炎症成分的疾病中尤其令人感兴趣。虽然外切体的分离和分析有助于了解这些多方面疾病背后的机制，但外切体也可能被开发为其治疗潜力。特别是，来自健康细胞的外切体已被发现有效地改变了癌细胞的转录组。尽管如此，目前还没有区分健康细胞和癌细胞来源的体液外切体的方法。在这个项目中，我们的目标是开发一种分离外切体和内切体的方法，以及一种区分外切体种群的方法。

Exosomes encapsulate and transport a wide variety of molecules generated by their cell-of-origin, a process now thought to be a form of cellular signaling. Exosome signaling is common across cell types and species, but it is of particular interest in diseases with an inflammatory component. While exosome isolation and analysis is useful to understanding the mechanisms behind these multifaceted diseases, exosomes may also be exploited for their therapeutic potential. In particular, exosomes derived from healthy cells have been found to effectively alter the transcriptome of cancer cells. Nonetheless, there are no methods for differentiating between healthy- and cancer-cell derived exosomes in bodily fluids. In this project, we aim to develop a method for isolating exosomes and oncosomes, coupled with a method for the differentiation of exosome populations.