Cefalu: Project 3; Moringa Isothiocyanates for Metabolic Resilience Moringa (Raskins)

G.计划摘要/摘要：项目3 这个项目的首要目标是测试异硫氰酸酯(ITCs)在叶子中产生的假设 辣木(Moringa Oleifera Lam)提供了一种有效、化学稳定和实用的不稳定替代品 十字花科(十字花科)植物产生的挥发性ITCs，用于增强弹性和 维持正常新陈代谢。 与十字花科中的“超级食品”相关的植物生产的ITC是最多的 从植物中分离出来的生物活性化合物。然而，在十字花科植物中形成的ITCs，如西兰花，共享 固有的耐热性和化学不稳定性使其在人类医学中的使用大大复杂化。 有趣的是，辣木(MICs)含有额外的糖残留物，赋予更大的稳定性和 在室温下表现为固体。稳定的ITC在自然界中极其罕见，几乎没有被探索过 有益的十字花科ITCs的替代品。我们开发了一种简单的一步提取/生物转化 一种有效地将辣木硫代葡萄糖酸盐转化为MIC的方法，从而得到辣木浓缩物(MC) 含有3%的麦克风。我们的初步数据表明，MC及其孤立的MIC具有潜在的抗 具有发炎、抗糖尿病、抗肥胖等功效。 拟议项目的早期阶段将集中于辣木提取物的优化、表征和 制定质量标准和植物认证标准(目标1)。后续研究将集中于 用GLOBAL研究其生物利用度和作用的分子和生理机制 基于代谢串扰、肠道微生物组和转录组学的方法(目标2和3)。通过关闭 与植物学和综合生物学核心的整合，并与我们拥有的其他项目充分合作 设计这些目的是为了测试增强的稳定性，结合高生物利用度和生物活性， 使Moringa ITCs和MC成为保持和提高韧性的有前景的植物学试剂 健康的新陈代谢和肠道微生物群。

G. Program Summary/Abstract: Project 3 The overarching goal of this project is to test the hypothesis that isothiocyanates (ITCs) produced in the leaves of moringa (Moringa oleifera Lam) provide an effective, chemically stable and practical alternative to instable and volatile ITCs produced by plants of the crucifer family (Brassicaceae) for building resiliency and maintaining normal metabolism. Plant-produced ITCs associated with “superfoods” in the crucifer family are among some of the most biologically active compounds isolated from plants. However, ITCs formed in crucifers, such as broccoli, share inherent thermolability and chemical instability that greatly complicate their use in human medicine. Interestingly, moringa ITCs (MICs) contain an additional sugar residue, conferring much greater stability and appear as solids at room termperature. Stable ITCs are extremely rare in nature and virtually unexplored as alternatives to beneficial crucifer ITCs. We have developed a simple, single-step extraction/biotransformation method which effectively converts moringa glucosinolates into MICs resulting in a moringa concentrate (MC) containing 3% MICs. Our preliminary data have shown that MC and its isolated MICs have potential anti- inflammatory, anti-diabetic and anti-obesity effects. Early stages of the proposed project will concentrate on moringa extract optimization, characterization and establishment of quality standards and botanical authentication criteria (Aim 1). Subsequent research will focus on the molecular and physiological mechanisms involved in its bioavailability and action using global approaches based on metabolic crosstalk, gut microbiome and transcriptomics (Aim 2 & 3). Through close integration with the Botanical and Integrative Biology Cores and in full collaboration with other projects we have designed these aims to test whether the enhanced stability, combined with high bioavailability and bioactivity, makes moringa ITCs and MC promising botanical agents for maintaining and promoting resilience based on healthy metabolism and gut microbiome.