Pluripotent Stem Cell and Organoid Core

项目摘要 消化健康中心（DHC）多能干细胞和类器官核心的总体目标是 提供专注于消化系统疾病研究的新的尖端技术， 来模拟人类疾病。核心通过四个相辅相成的目标来实现这一目标。在 核心的第一个目标是“为DHC研究者提供经过质量测试的PSC和PSC衍生的GI组织”， 向用户提供经过质量测试的人类多能干细胞（PSC）和PSC衍生的肠和胃 类器官这些是新颖的3D微型器官，可以进行与生理学和病理学相关的研究 与人类有关。在第二个目标中，“为DHC研究者生成活检衍生的人类类肠”， 核心也为研究者提供了产生胃、肠和结肠“类肠”的机会 健康或患病的受试者。在第三个目标“导出和质量测试疾病特异性诱导的PSC”中， 核心应用完善的转染、培养和表型分析方案来产生可诱导的PSC 从正常和患病的受试者中分离多能干细胞（iPSC），以促进疾病发病机制的研究，药物筛选， 等为了能够在实验测定中跟踪和可视化这些细胞，Core还提供PSC编辑功能。 以及根据研究者的需要建立新细胞系的筛选服务。在第四个目标中， 赞助关于PSC和类器官技术的培训课程和讲习班，”核心定期举行会议， 关于PSC培养的基本和先进技术，iPSC的产生，以及人类 消化组织类器官这些新技术使DHC研究人员能够研究 使用多细胞实验系统的疾病，这些系统具有正常人和患病人的直接“谱系” 组织（包括不同成熟阶段）。服务的提供得到精简和集中， 并让调查人员探索新的合作项目。

PROJECT SUMMARY The overall goal of the Pluripotent Stem Cell and Organoid Core of the Digestive Health Center (DHC) is to provide new, cutting edge technologies that are focused on digestive disease research and enable the use of tissue organoids to model human disease. The Core pursues this goal with four complementary aims. In the first aim “to provide DHC investigators with quality tested PSCs and PSC-derived GI tissues,” the Core makes available to users quality-tested human pluripotent stem cells (PSCs) and PSC-derived intestinal and gastric organoids. These are novel 3D-miniature organs that enable studies related to physiology and pathobiology relevant to humans. In the second aim “to generate biopsy-derived human enteroids for DHC investigators,” the Core also provides investigators with the opportunity to generate gastric, intestinal, and colonic “enteroids” from healthy or diseased subjects. In the third aim “to derive and quality test disease-specific induced PSC,” the Core applies well-established transfection, culture, and phenotyping protocols to generate inducible PSCs (iPSCs) from normal and diseased subjects to facilitate studies of pathogenesis of disease, drug screening, etc. To be able to track and visualize these cells in experimental assays, the Core also provide PSC-editing and screening services to establish novel cell lines based on investigators' needs. And in the fourth aim “to sponsor training courses and workshops on PSC and organoid technology,” the Core holds regular sessions on basic and advanced techniques for PSC culture, generation of iPSCs, and generation and use of human digestive tissue organoids. These novel technologies empower DHC investigators to study mechanisms of disease using multi-cellular experimental systems that have direct “lineage” to normal and diseased human tissues (including at different stages of maturation). The delivery of services is streamlined and centralized, and positions investigators to explore new collaborative projects.