Virology Core

摘要 这个新的科学核心的总体目标是提供集中的流感病毒学支持，以增强 该计划的总体结构-功能分析和免疫原发现活动。具体地说，核心B， 位于杜克大学的NIAID-区域生物遏制实验室(RBL)将提供全面的流感 病毒种群繁殖和高通量结合和中和分析支持。杜克大学的RBL是 完全批准BSL2、BSL3、选择剂和高途径禽流感风险组3的工作，并作为 杜克大学用联邦资金建造的多计划研究支持设施，以支持NIAID 生物防御议程或NIAID批准的其他生物医学研究活动。这些独特的服务不是 可通过杜克大学或哈佛大学的其他机构获得。拟议的核心B活动将具体用于 本次续签的所有项目第01页。病毒学核心的目标是：1)流感病毒株收集-开发， 维护和提供广泛的纯化流感病毒；以及2)流感体液反应 监测-执行集中的流感HA结合和微量中和分析(例如，人，小鼠， 和NHP标本)。纳入这一新的病毒学核心将提供一套通用的试剂和 对所有三个项目的宿主免疫反应进行机理分析的分析。对核心目标和 总体目标是持续致力于开发新的分析方法、质量控制监测和 与所有项目负责人协商，以预测和满足保险箱中调查小组的需求， 循规蹈矩，办事高效。

Abstract The overall goal of this new scientific core is to provide centralized influenza virology support to enhance the overall structure-function analysis and immunogen discovery activities of the program. Specifically, Core B, housed in the NIAID-Regional Biocontainment Laboratory (RBL) at Duke, will provide comprehensive influenza virus stock propagation and high-throughput binding and neutralization assay support. The RBL at Duke is fully approved for BSL2, BSL3, Select Agent and high path avian influenza Risk Group 3 work and is as a multi-disiplanary research support facility at Duke constructed with federal funds to support the NIAID biodefense agenda or other NIAID approved biomedical research activities. These unique services are not available through other facilities at Duke or Harvard. Proposed Core B activities will be specifically utilized by all projects in this renewal P01. The aims of the Virology Core are: 1) Influenza Strain Collections - develop, maintain and provide a broad panel of purified influenza viruses; and 2) Influenza Humoral Response Monitoring - perform centralized influenza HA binding and microneutralization assays (e. g. human, mouse, and NHP specimens). The inclusion of this new Virology Core will provide a common set of reagents and assays for mechanistic analysis of host immune response for all three projects. Critical to the Core aims and overall goal is an ongoing commitment to development of new assays, quality control monitoring and consultation with all project leaders to anticipate and meet the needs of the investigative teams in a safe, compliant and efficient manner.