Core 1: Biospecimen Core

项目总结/摘要 生物标本核心将继续提供高质量的注释标本和专家病理学 咨询，以加强案例GI孢子调查员的项目。这一进程将继续下去， 核心成员和单个项目之间长期、成功的合作 investigators.在这些合作的过程中，核心已经开发了一个广泛的生物标本， 来自13,000多名患者的档案。以前，核心提供高质量的注释标本 导致发现15-前列腺素脱氢酶作为结肠中重要的肿瘤抑制基因 致癌作用-项目1的基础;非裔美国人结肠癌15基因组-项目的基础 2;甲基化波形蛋白作为巴雷特食管的生物标志物-项目3的基础;以及 PIK 3CA突变结肠癌中独特的谷氨酰胺代谢-项目4的基础。核心还提供了 对SPORE的职业提升和试点项目计划的重大支持。芯部具有 来自4，800多名患者的结肠肿瘤的广泛档案，包括2，292例已知的原发性结肠癌 病理分期和基于数据库的临床随访以及大量的癌症转移。冷冻 来自这些病例中的1053例的材料也与匹配的正常对照一起储存。核心也有350个 食管癌和Barrett食管石蜡标本以及134例冷冻食管标本。 这些样品中的许多已经过广泛的分子分析， 问题研究核心与病例综合癌症中心合作，为SPORE提供广泛的 以具有成本效益的方式使用各种技术和工艺。核心将组织所有潜在的GI 生物标本的采购和分发给SPORE研究人员，并提供其现有的 组织档案资源。核心将通过识别感兴趣的组织来支持本申请的项目 从核心档案和预期应计中向研究者提供。核心将提供组织病理学检查 组织切片的质量控制，沿着项目特定形态学病例审查，并将监督和 提供与这些组织相关的临床结局的纵向随访。核心还将提供组织 感兴趣的组织靶点的微阵列切片，免疫组织化学和原位杂交的专业知识 评估和样品适合特定的研究。主要目标是提供一种资源， 对各个项目的转化目标和终点做出重大贡献，并促进 与其他GI SPORE合作。

PROJECT SUMMARY/ABSTRACT The Biospecimen Core will continue to provide high quality annotated specimens and expert pathology consultation to enhance the projects of Case GI SPORE investigators. This process will continue the longstanding, successful, collaborations between the members of the Core and the individual project investigators. During the course of these collaborations the Core has developed an extensive biospecimen archive derived from over 13,000 patients. Previously, the Core has supplied high quality annotated specimens leading to the discovery of 15-Prostaglandin Dehydrogenase as an important tumor suppressor gene in colon carcinogenesis – the basis of Project 1; the African American colon cancer 15 gene-panel – the basis of Project 2; methylated vimentin as a biomarker of Barrett's Esophagus – the basis of Project 3; and the discovery of unique glutamine metabolism in PIK3CA mutated colon cancers – the basis of Project 4. The Core also provided significant support to the SPORE's Career Enhancement and Pilot Projects Programs. The Core has an extensive archive of colon neoplasms from over 4,800 patients including 2,292 primary colon cancers of known pathologic stage and databased clinical follow-up as well as a substantial number of cancer metastases. Frozen material from 1053 of these cases is also banked with matched normal controls. The Core also has 350 esophageal cancers and Barrett's esophagus paraffin specimens as well as 134 frozen esophageal specimens. Many of these samples have undergone extensive molecular analysis – enabling sophisticated translational studies. The Core works with the Case Comprehensive Cancer Center to make available to the SPORE a wide variety of techniques and technologies in a cost effective manner. The Core will organize all prospective GI biospecimen procurements and distribution to SPORE investigators as well as provide access to its existing tissue archive resource. The Core will support the projects of this application by identification of tissues of interest to investigators both from the Core archive and from prospective accrual. The Core will provide histopathological quality control for tissue sections, along with project specific morphology case reviews and will oversee and provide longitudinal follow-up of clinical outcomes linked to these tissues. The Core will also provide tissue microarray sections for tissue targets of interest, expertise in immunohistochemistry and in situ hybridization assessment and samples appropriate for specific studies. The primary objective is to provide a resource that contributes significantly to the translational goals and endpoints of the individual projects and to facilitate cooperation between other GI SPOREs.