ShEEP request for a Zeiss LSM 880 with Airyscan confocal system

通过此应用程序，我们向ORD共享设备评估计划(SHEEP)申请资金 购买新的超分辨率共焦显微镜系统作为共享资源存放在 位于圣地亚哥退伍军人医学中心6楼的神经学项目实验室。具体来说， 我们寻求购买卡尔蔡司美国公司生产的带有Airycan的LSM 880型号。 (纽约州桑伍德市)此次收购的理由有三个。第一，当前的共焦显微镜 可通过圣地亚哥VAMC显微成像核心设备(蔡司LSM 510)和 神经修复中心(奥林巴斯FV1000)都有15年以上的历史，都超过了他们的运作 截至2016年9月的生命周期已过时，不再受制造商支持。vbl.去，去 未来，部件和维护将不再可用，这些显微镜，这是关键的 许多退伍军人管理局调查人员的研究目标将变得无法使用。第二，我们能够 蔡司购买LSM 880的限时20%折价优惠的优势 AiryScan系统。第三，配备AiryScan的LSM 880具有许多高级功能，使其在 共焦显微镜的尖端技术，包括任何其他技术所不具备的功能 制造商。因此，购买配备AiryScan共聚焦显微镜系统的LSM 880将 极大地增强了许多退伍军人管理局调查人员可用的成像能力，并支持 苛刻的研究目标。这必将刺激新的研究项目的发展和 圣地亚哥VAMC调查人员之间的新合作，该中心有长期的 卓越的研究成果，造福退伍军人健康。 该仪器的主要用户和次要用户的研究组合非常多样化，包括 研究脊髓损伤和神经修复的再生方法的项目以及研究 研究衰老的细胞和分子生物学，阿尔茨海默病的基因治疗 至于心脏病，脑外伤，精神病学，心脏病学，麻醉学， 神经退行性变、干细胞生物学、糖尿病和失明。使用最先进的共焦 显微镜工具和技术对所有这些研究都至关重要，因为它们都涉及收集 跨越大空间、时间和光谱动态的大量定量图像信息 从具有最高分辨率和速度的各种活的和固定的生物样本 有可能。具体地说，配备Airycan的LSM 880将是至关重要的研究小组和项目 包括： 马克·图辛斯基博士、约翰·布罗克博士、保罗·卢博士、布莱恩·海德博士、罗伯特·里斯曼博士、海马尔·帕特尔博士、 Thomas Hnasko博士、Allen Ryan博士、Steven Wagner博士、Dhakshin Ramanathan博士和Veronica博士 舒巴耶夫、柯克·哈蒙德博士和埃夫龙·罗森茨韦格博士。 因此，使用AiryScan收购LSM 880不仅将在SAN上保持共焦显微镜 迭戈退伍军人医疗中心。相反，这项尖端技术将有助于确保我们保持 我们各自领域的领导者站在研究的前沿，以改善退伍军人的健康。

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a new super resolution confocal microscope system as a shared resource to be housed in the Neurology Program laboratories on the 6th floor of the San Diego VA Medical Center. Specifically, we seek to obtain a model LSM 880 with Airyscan manufactured by Carl Zeiss USA, Inc. (Thornwood, NY). The rationale for this purchase is threefold. First, Current confocal microscope systems available through the San Diego VAMC Microimaging Core Facility (Zeiss LSM 510) and the Center for Neural Repair (Olympus FV1000) are both over 15 years old, are well over their operational lifetimes and as of September 2016 are obsolete and no longer supported by the manufacturers. Going forward, parts and maintenance will no longer be available and these microscopes, which are critical to the research goals of many VA investigators, will become unusable. Second, we are able to take advantage of a time-limited 20% trade-in offer from Zeiss for the purchase of the LSM 880 with Airyscan system. Third, the LSM 880 with Airyscan has many advanced features that place it at the cutting edge of confocal microscopy, including features that are not available from any other manufacturer. Therefore, the acquisition of an LSM 880 with Airyscan confocal microscopy system will dramatically enhance the imaging capabilities available to many VA investigators and support the most demanding research goals. This will certainly stimulate the development of new research projects and new collaborations among investigators at the San Diego VAMC, which has a long-standing record of excellence in research to benefit Veterans health. The research portfolio of the major and minor users for this instrument is very diverse and includes projects studying regenerative approaches to spinal cord injury and neural repair as well as studies investigating the cellular and molecular biology of ageing, gene therapy for Alzheimer's disease as well as for cardiac disease, traumatic brain injury, psychiatry, cardiology, anesthesiology, neurodegeneration, stem cell biology, diabetes and blindness. Access to the most advanced confocal microscopy tools and techniques is critical to all of these studies, as they all involve the collection of large amounts of quantitative image information across a large spatial, temporal and spectral dynamic range from a diverse set of living and fixed biological samples with the highest resolution and speed possible. Specifically, research groups and projects for which the LSM 880 with Airyscan will be critical are: Dr Mark Tuszynski, Dr John Brock, Dr Paul Lu, Dr Brian Head, Dr Robert Rissman, Dr Hemal Patel, Dr Thomas Hnasko, Dr Allen Ryan, Dr Steven Wagner, Dr Dhakshin Ramanathan and Dr Veronica Shubayev, Dr Kirk Hammond and Dr Ephron Rosenszweig. Thus, acquisition of an LSM 880 with Airyscan will not merely maintain confocal microscopy at the San Diego VA Medical Center. Rather, this cutting edge technology will help to ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran's health.