Transgenic Animal Shared Facility

分子分析/翻译组 转基因动物共享因子（TASF） 摘要 UAB转基因动物共享设施（TASF）是UAB综合癌症中心的重要组成部分。 中心及其研究企业。转基因小鼠模型仍然是最容易驾驭的 哺乳动物系统，以检查与人类疾病相关的已鉴定遗传变异的作用，以及 为开发新疗法创造急需的转化模型。该共享设施提供 为转基因动物模型的开发提供独特的服务，并拥有长期的跟踪记录， 出色的服务。具体目标包括： 1.提供高效、经济的转基因和基因工程动物模型生产， 综合癌症中心的研究人员。为了加强癌症研究，该中心将继续其富有成效的工作， 通过将基因构建体显微注射到受精小鼠卵中开发新型小鼠模型的历史， 胚胎干细胞进入囊胚，此外，现在还提供服务，创造大鼠和斑马鱼的遗传模型。 2.在动物模型开发和支持方面提供额外的基本服务。发展和 动物模型的管理将包括：TALEN和CRISPR合成和基因靶向， 冷冻保存胚胎和精子，胚胎再衍生以产生无病原体的动物，辅助 再现技术（例如，体外受精（IVF）、超数排卵和胚胎移植）。 3.提供专家咨询服务，帮助研究者设计最有效的转基因和基因 靶向构建体、分子鉴定策略和产生所需的育种策略 转基因实验动物和适当的对照动物。 所提供的服务不通过任何其他机制提供，除了外部的商业或学术 无法提供该设施提供的全套服务或成本过高的替代方案 费用和/或时间障碍。

MOLECULAR ANALYSIS / TRANSLATION GROUP TRANSGENIC ANIMAL SHARED FACILITY (TASF) ABSTRACT The UAB Transgenic Animal Shared Facility (TASF) is a vital component of the UAB Comprehensive Cancer Center, and its research enterprise. Genetically modified mouse models continue to be the most tractable mammalian system to examine the role of an identified genetic variant associated with human disease, as well as creating much needed translational models for developing novel therapeutics. This shared facility provides unique services for the development of genetically modified animal models and has a long track record of outstanding service. The specific aims include: 1. Provide efficient, cost-effective production of transgenic and genetically-engineered animal models for Comprehensive Cancer Center investigators. To enhance cancer research the core will continue its productive history of developing novel mouse models by microinjection of gene constructs into fertilized mouse eggs or ES cells into blastocysts, and additionally now offers services to create both rat and zebrafish genetic models. 2. Provide additional essential services in animal model development and support. Development and management of animal models will include: TALEN and CRISPR synthesis and gene targeting, cryopreservation of embryos and sperm, embryo re-derivation to produce pathogen-free animals, assisted reproduction techniques (e.g., in vitro fertilization [IVF], superovulation, and embryo transfer). 3. Provide expert consultation services to help investigators design the most effective transgene and gene targeting constructs, strategies for molecular identification, and breeding strategies required for generating genetically modified experimental animals and appropriate control animals. The services provided are not available through any other mechanism except external commercial or academic alternatives that do not supply the complete set of services provided by this facility, or are prohibitively costly in terms of both expense and/or time impediments.