Cluster A: 2 Viral Vector Core

病毒载体核心(VVC)被整合到针对该疾病研究的多个癌症项目中 疫苗的过程、治疗和临床前开发。VVC员工积极参与 基因转移技术在癌症领域的发展。与多名调查人员的互动来自 不同的学科允许思想、技术进步和矢量创新的交叉 设计。VVC的总体目标是支持研究人员使用基因转移技术， 包括与PI和工作人员协商、开发新的载体、协作测试载体 产生的功能和纯度，最后例行准备，包括质量控制。VVC员工和 研究人员在载体设计和生成的所有阶段都保持密切联系，VVC充当 既有基因转移研究的研发设施，也有常规载体的服务设施 准备工作。作为服务的一部分，VVC将提供纯化和浓缩的制剂 重组腺病毒、腺相关病毒、牛痘病毒、杆状病毒和逆转录病毒(包括慢病毒)。 这一设施提供了获得标准细胞系、表达质粒和重组报告病毒库存的途径 向量。 VVC的主要职责是：准备重组载体；进行相关的质量控制； 传播媒介；维护可供使用的媒介种群数据库；维护表达数据库 载体；根据需要开发新的表达载体；开发新的载体生产方法；以及协助 在新型载体的设计和开发中。 VVC满足了众多对基础和应用感兴趣的外部癌症研究人员的需求 利用基因转移载体进行研究，出于几个原因，这一点很重要。首先，与许多人并驾齐驱 不同的基因转移载体、方法和应用，VVC工作人员的科学专业知识是 得到了加强。第二，为更广泛的用户提供服务改善和促进了校际交流 重点致力于开发改进的病媒和投递方法。最后，一系列新的想法 来自大学内外，并通过我们的顾问，确保HCCC社区 有权接触或了解“最先进的技术”。

The Viral Vector Core (VVC) is integrated into multiple cancer projects directed at the study of the disease process, therapy and the pre-clinical development of vaccines. VVC staff is active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The VVC's overall objective is to support investigators in the use of gene transfer technologies, including consultation with the PI and staff, development of novel vectors, collaborative testing of vectors generated for function and purity, and finally routine preparation including quality control. VVC staff and investigators are in close contact through all phases of vector design and generation, and the VVC serves as both a research and development facility for gene transfer studies and a service facility for routine vector preparations. As a part of the service the VVC will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus, vaccinia, baculovirus and retrovirus (including lentivirus). This facility provides access to standard cell lines, expression plasmids, and stock of recombinant reporter viral vectors. The main responsibilities of the VVC are to: prepare recombinant vectors; perform relevant quality control; disseminate vectors; maintain a database of vector stocks available for use; maintain a database of expression vectors; develop new expression vectors as needed; develop novel methods for vector production; and assist in the design and development of novel vectors. The VVC serves the needs of numerous outside cancer investigators interested in both basic and applied research with gene transfer vectors, and is important for several reasons. First, by keeping abreast of many different gene transfer vectors, approaches, and applications, the scientific expertise of the VVC staff is strengthened. Second, serving a broad scope of users improves and fosters inter-collegiate communication with focused efforts at developing improved vectors and delivery methods. Finally, a continuum of new ideas from both outside and within the university, and through our consultants, insures that the HCCC community has access to, or is aware of, the “state of the art”.